US 11,919,927 C1 (263rd)
Prefusion-stabilized hMPV F proteins
Jason McLellan, Austin, TX (US); Ching-Lin Hsieh, Austin, TX (US); Scott Rush, Austin, TX (US); and Nianshuang Wang, Austin, TX (US)
Filed by BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
Assigned to BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
Supplemental Examination Request No. 96/050,094, May 29, 2025.
Reexamination Certificate for Patent 11,919,927, issued Mar. 5, 2024, Appl. No. 18/296,771, Apr. 6, 2023.
Application 96/050,094 is a continuation of application No. PCT/US2021/053944, filed on Oct. 7, 2021.
Claims priority of provisional application 63/089,978, filed on Oct. 9, 2020.
This patent is subject to a terminal disclaimer.
Ex Parte Reexamination Certificate from Reexamination Ordered under 35 U.S.C. 257 issued on Jan. 20, 2026.
Int. Cl. C07K 14/08 (2006.01); A61K 39/12 (2006.01); A61K 39/39 (2006.01); A61P 31/14 (2006.01); C07K 14/005 (2006.01); A61K 39/00 (2006.01); A61K 39/155 (2006.01)
CPC C07K 14/08 (2013.01) [A61K 39/12 (2013.01); A61K 39/39 (2013.01); A61P 31/14 (2018.01); C07K 14/005 (2013.01); A61K 39/155 (2013.01); A61K 2039/55561 (2013.01); A61K 2039/575 (2013.01); C12N 2760/18322 (2013.01); C12N 2760/18334 (2013.01)]
AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT:
Claim 1 is determined to be patentable as amended.
Claims 2-30 were not reexamined.
1. An engineered protein comprising a metapneumovirus (MPV) F protein ectodomain having at least 90% identity to amino acids 19-489 of any one of SEQ ID NOs: 4-7, said engineered protein comprising one or more of:
paired cysteine substitutions corresponding to A140C and A147C,
paired cysteine substitutions corresponding to L110C and N322C,
paired cysteine substitutions corresponding to T127C and N153C,
substitutions at positions corresponding to L219 and/or V231,
substitutions corresponding to L219K and V231I,
paired cysteine substitutions corresponding to T127C and N153C and paired cysteine
substitutions corresponding to T365C and V463C,
a substitution corresponding to G366S,
substitutions corresponding to T127C, N153C, A185P, T365C, V463C, L219K, and V231I,
substitutions corresponding to T127C, N153C, A185P, T365C, V463C, L219K, V231I, and RQSR (residues corresponding to positions 99-102 of any one of SEQ ID NOs: 4-7) to RRRR (SEQ ID NO: 10),
substitutions corresponding to T127C, N153C, T365C, V463C, L219K, and V231I,
substitutions corresponding to L110C, T127C, A140C, A147C, N153C, A185P, L219K, V231I, N322C, T365C, E453Q, and V463C,
substitutions corresponding to L110C, T127C, A140C, A147C, N153C, A185P, L219K, V231I, N322C, T365C, E453Q, V463C, and RQSR (residues corresponding to positions 99-102 of any one of SEQ ID NOs: 4-7) to RRRR (SEQ ID NO: 10), and
substitutions corresponding to L110C, T127C, A140C, A147C, N153C, L219K, V231I, N322C, T365C, E453Q, and V463C, relative to the sequence of any one of SEQ ID NOs: 4-7.