US 10,775,376 C1 (13,131st)
Methods for assaying cellular binding interactions
Anupam Singhal, Mississauga (CA); Carl L. G. Hansen, Vancouver (CA); John W. Schrader, Vancouver (CA); Charles A. Haynes, Vancouver (CA); and Daniel J. Da Costa, Pitt Meadows (CA)
Filed by The University of British Columbia, Vancouver (CA)
Assigned to THE UNIVERSITY OF BRITISH COLUMBIA, Vancouver (CA)
Reexamination Request No. 90/019,666, Sep. 17, 2024.
Reexamination Certificate for Patent 10,775,376, issued Sep. 15, 2020, Appl. No. 16/746,540, Jan. 17, 2020.
Application 90/019,666 is a continuation of application No. 16/579,561, filed on Sep. 23, 2019, granted, now 10,578,618.
Application 16/579,561 is a continuation of application No. 16/290,751, filed on Mar. 1, 2019, granted, now 10,466,241, issued on Nov. 5, 2019.
Application 16/290,751 is a continuation of application No. 16/129,555, filed on Sep. 12, 2018, granted, now 10,274,494, issued on Apr. 30, 2019.
Application 16/129,555 is a continuation of application No. 14/879,791, filed on Oct. 9, 2015, granted, now 10,107,812, issued on Oct. 23, 2018.
Application 14/879,791 is a continuation of application No. 13/184,363, filed on Jul. 15, 2011, granted, now 9,188,593, issued on Nov. 17, 2015.
Claims priority of provisional application 61/365,237, filed on Jul. 16, 2010.
Ex Parte Reexamination Certificate issued on Jan. 2, 2026.
Int. Cl. G01N 33/569 (2006.01); B01L 3/00 (2006.01); G01N 33/577 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/56966 (2013.01) [B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); G01N 33/577 (2013.01); G01N 33/582 (2013.01); G01N 33/6854 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0481 (2013.01)]
OG exemplary drawing
AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT:
The patentability of claims 1, 12 and 18-20 is confirmed.
Claims 2-11, 13-17 and 21-27 were not reexamined.
1. A method of assaying for a binding interaction between an antibody secreted by a single antibody secreting cell (ASC) and an antigen, the method comprising:
retaining the single ASC within a chamber having a volume of less than 500 pL, a solid wall, and an aperture that defines an opening of the chamber;
incubating the single ASC within the chamber to produce a secreted antibody;
bringing a first fluid volume comprising the antigen in fluid communication with the secreted antibody;
exposing the secreted antibody to a removeable capture substrate, wherein the removeable capture substrate is in fluid communication with the secreted antibody and wherein the removeable capture substrate is operable to bind the secreted antibody;
incubating the secreted antibody with the removeable capture substrate to produce a bound antibody; and
measuring a binding interaction between the secreted antibody and the antigen.