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1

DIRECTED MOLECULAR EVOLUTION OF MACROMOLECULES (E.G., RNA, DNA, PROTEINS, ETC.)

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METHOD SPECIALLY ADAPTED FOR IDENTIFYING A LIBRARY MEMBER

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Identifying a library member by its fixed physical location on a support or substrate

4

Identifying a library member by means of a tag, label, or other readable or detectable entity associated with the library member (e.g., decoding process, etc.)

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Using an iterative deconvolution technique

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Direct analysis of a library member, per se, by a physical method (e.g., spectroscopy, etc.)

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METHOD OF SCREENING A LIBRARY

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In silico screening

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By measuring the ability to specifically bind a target molecule (e.g., antibody-antigen binding, receptor-ligand binding, etc.)

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By measuring the effect on a living organism, tissue, or cell

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By measuring catalytic activity

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By measuring a physical property (e.g., mass, etc.)

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LIBRARY, PER SE (E.G., ARRAY, MIXTURE, IN SILICO, ETC.)

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Library contained in or displayed by a micro-organism (e.g., bacteria, animal cell, etc.) or library contained in or displayed by a vector (e.g., plasmid, etc.) or library containing only micro-organisms or vectors

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Library containing only organic compounds

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Nucleotides or polynucleotides, or derivatives thereof

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RNA or DNA which encodes proteins (e.g., gene library, etc.)

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Peptides or polypeptides, or derivatives thereof

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Saccharides or polysaccharides, or derivatives thereof

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Macromolecular compounds (e.g., synthetic resin, rubber, etc.)

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Metal-containing organic compounds

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Library containing only inorganic compounds or inorganic materials

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METHOD OF CREATING A LIBRARY (E.G., COMBINATORIAL SYNTHESIS, ETC.)

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In silico or mathematical conception of a library

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Using a dynamic combinatorial chemistry technique

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Biochemical method (e.g., using an enzyme or whole viable micro-organism, etc.)

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Liquid-phase synthesis (i.e., wherein all library building blocks are in liquid phase or in solution during library creation) or particular method of cleavage from the liquid support used

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Involving an encoding step

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Using a particular method of attachment to the liquid support

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Solid-phase synthesis (i.e., wherein one or more library building blocks are bound to a solid support during library creation) or particular method of cleavage from the solid support used

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Involving an encoding step

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Using a particular method of attachment to the solid support

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APPARATUS SPECIALLY ADAPTED FOR USE IN COMBINATORIAL CHEMISTRY OR WITH A LIBRARY

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Integrated apparatus specially adapted for creating a library, screening a library, and identifying a library member

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Integrated apparatus specially adapted for both screening a library and identifying a library member

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Integrated apparatus specially adapted for both creating a library and identifying a library member

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Integrated apparatus specially adapted for both creating and screening a library

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For identifying a library member

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For screening a library

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For creating a library

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TAG OR LABEL SPECIALLY ADAPTED FOR COMBINATORIAL CHEMISTRY OR A LIBRARY (E.G., FLUORESCENT TAG, BAR CODE, ETC.)

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LINK OR SPACER SPECIALLY ADAPTED FOR COMBINATORIAL CHEMISTRY OR A LIBRARY (E.G., TRACELESS LINKER, SAFETY-CATCH LINKER, ETC.)

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MISCELLANEOUS

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