US 9,810,966 B2
Random access stimulated emission depletion (STED) microscopy
Paolo Bianchini, Genoa (IT); Peter Saggau, Houston, TX (US); and Alberto Diaspro, Genoa (IT)
Assigned to FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, Genoa (IT); and BAYLOR COLLEGE OF MEDICINE, Houston, TX (US)
Appl. No. 14/778,217
Filed by FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, Genoa (IT); and BAYLOR COLLEGE OF MEDICINE, Houston, TX (US)
PCT Filed Mar. 21, 2014, PCT No. PCT/IB2014/060024
§ 371(c)(1), (2) Date Sep. 18, 2015,
PCT Pub. No. WO2014/147590, PCT Pub. Date Sep. 25, 2014.
Claims priority of application No. TO2013A0229 (IT), filed on Mar. 22, 2013.
Prior Publication US 2016/0274439 A1, Sep. 22, 2016
Int. Cl. G02B 21/00 (2006.01); G02F 1/33 (2006.01); G02B 21/16 (2006.01); G02B 27/58 (2006.01); G01N 21/64 (2006.01); G02B 27/09 (2006.01)
CPC G02F 1/33 (2013.01) [G01N 21/6456 (2013.01); G02B 21/002 (2013.01); G02B 21/0036 (2013.01); G02B 21/0076 (2013.01); G02B 21/0092 (2013.01); G02B 21/16 (2013.01); G02B 27/58 (2013.01); G02B 27/09 (2013.01)] 8 Claims
OG exemplary drawing
 
1. Apparatus for irradiating a target material in order to excite elements of an excitable chemical species contained within said target material to an excited state, comprising
an optical scanning system comprising:
an optical system for guiding a first and a second light beam, and
a deflecting device for deflecting said first and second light beams in a directionally variable manner,
wherein said deflecting device comprises at least one acousto-optic deflector, and said optical system is arranged in such a way that said first and second light beams are counter-propagating through said at least one acousto-optic deflector, said at least one acousto-optic deflector being controllable for deflecting, simultaneously or in pulse sequence, said first and second light beams,
wherein said first light beam is constituted by a short-pulsed excitation beam and is adapted to excite said elements of an excitable chemical species to said excited state, and said second light beam is constituted by a long-pulsed depletion beam and is adapted to reduce the number of excited elements in said excited state, and
a directing system for directing said first and second light beams onto said target material in such a way that said first and second light beams form respective movable, partially overlapped, irradiation areas on said target material,
wherein the pulse length of the excitation beam is less than 1 ps, and wherein the pulse length of the depletion beam is longer than 50 ps.