US 9,810,895 B2
Biological observation apparatus
Hiroyuki Kimura, Tokyo (JP)
Assigned to OLYMPUS CORPORATION, Tokyo (JP)
Filed by Hiroyuki Kimura, Tokyo (JP)
Filed on May 25, 2010, as Appl. No. 12/786,750.
Claims priority of application No. 2009-130880 (JP), filed on May 29, 2009.
Prior Publication US 2010/0310139 A1, Dec. 9, 2010
Int. Cl. G06K 9/00 (2006.01); G02B 21/36 (2006.01); G02B 21/00 (2006.01)
CPC G02B 21/367 (2013.01) [G02B 21/002 (2013.01); G02B 21/008 (2013.01); G02B 21/0072 (2013.01); G02B 21/0076 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for creating an image of a moving live-cell specimen using an observation apparatus comprising a microscope system and an information storage unit, wherein the microscope system comprises a stage on which the live-cell specimen is mounted and an objective lens that collects observation light from the live-cell specimen while focusing illumination light thereon, the method comprising:
acquiring, with the microscope system, a micro image of the live-cell specimen;
acquiring, with the microscope system, a three-dimensional macro image of an observation region that includes the live-cell specimen, by varying a relative position of the objective lens and the live-cell specimen in an optical axis direction, wherein a viewing range of the microscope system when acquiring the macro image is larger than a viewing range of the microscope system when acquiring the micro image;
extracting identification information of the live-cell specimen included in the macro image by performing image processing on the macro image;
storing, in the information storage unit, identification information of the live-cell specimen which moves and which is observed as a target;
specifying, as a specified live-cell specimen, the live-cell specimen to which the extracted identification information corresponds in the identification information stored in the information storage unit;
calculating an amount of movement of the specified live-cell specimen included in the macro image; and
adjusting repeatedly, according to a movement of the live-cell specimen, a position of the viewing range of the microscope system in a direction that intersects an optical axis, based on the calculated amount of movement of the specified live-cell specimen, such that the specified live-cell specimen is included in the viewing range of the microscope system.