US 9,810,894 B2
Tracking and characterizing particles with holographic video microscopy
David G. Grier, New York, NY (US); Sang-Hyuk Lee, Bridgewater, NJ (US); and Fook C. Cheong, Brooklyn, NY (US)
Assigned to NEW YORK UNIVERSITY, New York, NY (US)
Filed by NEW YORK UNIVERSITY, New York, NY (US)
Filed on Jul. 29, 2014, as Appl. No. 14/446,306.
Application 14/446,306 is a continuation of application No. 12/740,628, granted, now 8,791,985, previously published as PCT/US2008/081794, filed on Oct. 30, 2008.
Claims priority of provisional application 61/001,023, filed on Oct. 30, 2007.
Claims priority of provisional application 61/073,959, filed on Jun. 19, 2008.
Prior Publication US 2014/0333935 A1, Nov. 13, 2014
This patent is subject to a terminal disclaimer.
Int. Cl. G02B 21/36 (2006.01); G01N 15/02 (2006.01); G01N 15/14 (2006.01); G01P 5/00 (2006.01); G01P 5/20 (2006.01); G03H 1/00 (2006.01); G01B 9/02 (2006.01); G01N 15/10 (2006.01); G01N 15/00 (2006.01); G03H 1/08 (2006.01); G03H 1/04 (2006.01)
CPC G02B 21/361 (2013.01) [G01B 9/02001 (2013.01); G01N 15/0227 (2013.01); G01N 15/10 (2013.01); G01N 15/1463 (2013.01); G01P 5/001 (2013.01); G01P 5/20 (2013.01); G03H 1/0005 (2013.01); G01N 2015/0038 (2013.01); G01N 2015/025 (2013.01); G01N 2015/1075 (2013.01); G03H 1/0866 (2013.01); G03H 2001/005 (2013.01); G03H 2001/0033 (2013.01); G03H 2001/0447 (2013.01); G03H 2001/0825 (2013.01); G03H 2240/56 (2013.01)] 14 Claims
OG exemplary drawing
 
11. A computer implemented system comprising:
a holographic microscope apparatus comprising a coherent light source with multiple discrete concurrent wavelengths of coherent light beams' a specimen stage, an objective lens, and an image collection device;
a computer module in communication with the holographic microscope apparatus and including a processor and memory, the memory receiving image data from the image collection device and further having a set of instructions for;
selecting multiple wavelengths for a laser;
scattering the laser's beam off the specimen to generate a scattered portion;
generating an interference pattern from an unscattered portion of the collimated laser beam and the scattered portion;
recording the interference pattern for subsequent analysis;
applying a scattering function to analyze the recorded interference pattern wherein the scattering function comprises a Lorenz-Mie function;
normalizing the interference pattern by dividing the interference pattern by a background form of interference pattern;
fitting a calculated hologram to the interference pattern; and
determining an estimate of the specimen's refractive index and radius from the fitted calculated hologram;
wherein the determining of the refractive index of the specimen comprises measuring refractive indices of the specimen at each of the multiple wavelengths.