US 9,810,707 B2
Chip-based flow cytometer type systems for analyzing fluorescently tagged particles
Adam Richard Schilffarth, Austin, TX (US); William R. Deicher, Austin, TX (US); John C. Carrano, Austin, TX (US); and Jesse C. Phillips, Austin, TX (US)
Assigned to LUMINEX CORPORATION, Austin, TX (US)
Filed by Adam Richard Schilffarth, Austin, TX (US); William R. Deicher, Austin, TX (US); John C. Carrano, Austin, TX (US); and Jesse C. Phillips, Austin, TX (US)
Filed on May 17, 2007, as Appl. No. 11/750,000.
Claims priority of provisional application 60/747,483, filed on May 17, 2006.
Prior Publication US 2007/0269345 A1, Nov. 22, 2007
Int. Cl. G01N 33/00 (2006.01); G01N 35/10 (2006.01); G01N 35/00 (2006.01); G01N 15/14 (2006.01); G01N 21/64 (2006.01)
CPC G01N 35/1095 (2013.01) [G01N 15/1484 (2013.01); G01N 35/0098 (2013.01); G01N 15/1459 (2013.01); G01N 2021/6439 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A portable system for processing and analyzing biological or environmental samples, comprising:
an automated assay preparation module configured to process a sample into a fluid assay with fluorescently tagged particles; and
a microfluidic analysis module coupled to the automated assay preparation module, wherein the microfluidic analysis module comprises a chip-based flow cytometer having:
a first input conduit for receiving the fluid assay;
a second distinct conduit for receiving a sheath fluid;
a fluid flow chamber coupled to the first and second input conduits, wherein the fluid flow chamber is configured to generate a fluid stream with the fluid assay confined within the sheath fluid;
a channel coupled to the fluid flow chamber for routing the fluid stream through the flow cytometer;
an illumination subsystem comprising a light source system and an optical system collectively configured to direct light toward an interrogation region of the channel;
a measurement subsystem comprising:
a collection system comprising collection optics configured to gather fluorescence emitted from the fluorescently tagged particles; and
an examination system for analyzing the collected fluorescence;
wherein the first conduit, second conduit, fluid flow chamber, channel, and at least some collection optics of the collection system are fixedly arranged within a substrate, and
wherein the light source system is configured such that individual light sources within the light source system direct light at different spots within the interrogation region, and wherein the collection system is configured such that the fluorescent light emitted from the fluorescently tagged particles at each of the different spots is collected by a different detector of the collection system.