US 9,810,694 B2
Identification of fully human antibodies for use in therapy and diagnosis of human diseases
Gennady Bratslavsky, Fayetteville, NY (US); and Ilya Tsimafeyeu, Mogilev (BY)
Filed by Gennady Bratslavsky, Fayetteville, NY (US); and Ilya Tsimafeyeu, Mogilev (BY)
Filed on Aug. 6, 2015, as Appl. No. 14/819,500.
Prior Publication US 2017/0038392 A1, Feb. 9, 2017
Int. Cl. G01N 33/68 (2006.01); G01N 33/574 (2006.01)
CPC G01N 33/6854 (2013.01) [G01N 33/57434 (2013.01); G01N 2500/10 (2013.01)] 8 Claims
 
1. A method for identification of therapeutic and diagnostic high-affinity fully human antibodies that have infiltrated a human tissue; said method comprising the steps of:
(a) obtaining and preparing said human tissue supernatants and/or lysates from patients and donors;
(b) contacting step (a) with an antigen of interest;
(c) identifying fully human antibody from step (b) by binding assay;
(d) Isotyping the fully human antibody and selecting the high-affinity fully human antibody from step (c);
(e) Evaluating of said selected high-affinity fully human antibody by treating tissue of interest with said selected high-affinity fully human antibody and evaluating its effect thereof;
(f) Establishing production and purification of a recombinant of said high-affinity fully human antibody for therapeutic and diagnostic use.