US 9,810,688 B2
Smart glass slide for microarrays
Nadezda Fomina, Fremont, CA (US); Christopher Johnson, San Carlos, CA (US); Sam Kavusi, Menlo Park, CA (US); and Habib Ahmad, Sunnyvale, CA (US)
Assigned to ROBERT BOSCH GMBH, Stuttgart (DE)
Filed by Robert Bosch GmbH, Stuttgart (DE)
Filed on Mar. 15, 2013, as Appl. No. 13/834,126.
Prior Publication US 2014/0274760 A1, Sep. 18, 2014
Int. Cl. G01N 33/543 (2006.01); C40B 60/00 (2006.01); C40B 30/04 (2006.01); C40B 40/10 (2006.01); G01N 27/327 (2006.01)
CPC G01N 33/5438 (2013.01) [G01N 27/3272 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A device for use in a biosensor, the device comprising a multisite array of test sites, the multisite array of test sites including:
(1) a support substrate, wherein the support substrate is glass or plastic; and
(2) a biomolecular interface layer that includes:
(i) a layer of immobilized polyethylene glycol (PEG) that covers the support substrate;
(ii) a plurality of Green Fluorescent Protein spots that are immobilized by attachment directly to the layer of immobilized PEG; and
(iii) a plurality of immobilized probes immobilized by attachment directly to the layer of immobilized PEG, wherein the plurality of immobilized probes are selected from the group consisting of a protein, a peptide, an antibody, an antigen, a nucleic acid, and a cell; wherein:
(a) each test site of the multisite array of test sites comprises:
(i) a single Green Fluorescent Protein spot of the plurality of Green Fluorescent Protein spots;
(ii) at least one working electrode that includes one or more counter-electrodes;
(iii) a first area of the support substrate that supports, and is covered by, the at least one working electrode, wherein the device is configured to repeatedly modulate a pH of the first area of the support substrate with the at least one working electrode; and
(iv) a second area of the support substrate;
(b) the biomolecular interface layer is arranged such that, with respect to each test site of the multisite array of test sites:
(i) a first portion of the single Green Fluorescent Protein spot covers the at least one working electrode that covers the first area of the support substrate; and
(ii) a second portion of the single Green Fluorescent Protein spot covers the second area of the support substrate without any working electrodes arranged between the second area of the support substrate and the second portion of the single Green Fluorescent Protein spot that covers the second area of the support substrate; and
(c) independently with respect to each test site of the multisite array of test sites:
(i) the device is configured to repeatedly perform the following:
(1) determine the pH level in the first area of the support substrate by detection of a fluorescence intensity of the first portion of the single Green Fluorescent Protein spot covering the at least one working electrode; and
(2) determine the pH level in the second area of the support substrate by detection of a fluorescence intensity of the second portion of the single Green Fluorescent Protein spot covering the second area of the support substrate; and
(ii) the pH level determined for the second area of the support substrate is a control by which the device is configured to determine when the pH level determined for the first area of the support substrate has reverted to a base level.