US 9,810,687 B2
Nanocomposites and methods of making same
Alexandru S. Biris, Little Rock, AR (US); Andrew G. Kumpuris, Little Rock, AR (US); and Zeid Nima, Little Rock, AR (US)
Assigned to BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US)
Filed by BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS, Little Rock, AR (US)
Filed on Apr. 10, 2015, as Appl. No. 14/683,929.
Application 14/683,929 is a continuation in part of application No. 14/513,744, filed on Oct. 14, 2014, granted, now 9,784,737.
Claims priority of provisional application 61/891,006, filed on Oct. 15, 2013.
Claims priority of provisional application 62/082,513, filed on Nov. 20, 2014.
Prior Publication US 2016/0077090 A1, Mar. 17, 2016
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); G01N 21/65 (2006.01)
CPC G01N 33/54346 (2013.01) [G01N 21/658 (2013.01); G01N 2800/28 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A nanoagent comprising at least one nanocomposite, the nanocomposite comprising:
at least one gold nanorod;
a silver layer coated on an outer surface of the gold nanorod, the silver layer comprising silver nanoparticles;
a Raman reporter molecule layer coated on the silver layer, wherein the Raman reporter molecule layer comprises the Raman reporter molecules that are detectable by surface enhanced Raman spectroscopy (SERS);
a pegylated layer coated on the Raman reporter molecule layer, comprising at least one of thiolated polyethylene glycol (HS-PEG), thiolated polyethylene glycol acid (HS-PEG-COOH) and HS-PEG-NHx; and
an active layer conjugated to the pegylated layer, the active layer comprising at least one of:
a targeting molecule configured to bind to a target of interest; and
a functional molecule configured to interact with the target of interest,
wherein the at least one nanocomposite comprises a first nanocomposite, a second nanocomposite, a third nanocomposite, and a fourth nanocomposite;
wherein the Raman reporter molecule layer of the first nanocomposite comprises 4-mercaptobenzoic acid (4MBA);
wherein the Raman reporter molecule layer of the second nanocomposite comprises p-aminothiophenol (PATP);
wherein the Raman reporter molecule layer of the third nanocomposite comprises p-nitrothiophenol (PNTP);
wherein the Raman reporter molecule layer of the fourth nanocomposite comprises 4-(methyl sulfanyl)thiophenol (4MSTP); and
wherein SERS signal corresponding to each of the first, second, third and fourth nanocomposites is represented by a predetermined color.