US 9,810,685 B2
High-throughput platform comprising microtissues perfused with living microvessels
Steven C. George, Irvine, CA (US); Christopher C. W. Hughes, Irvine, CA (US); Abraham P. Lee, Irvine, CA (US); Monica Moya, Irvine, CA (US); and Yu-Hsiang Hsu, Pasadena, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by Steven C. George, Irvine, CA (US); Christopher C. W. Hughes, Irvine, CA (US); Abraham P. Lee, Irvine, CA (US); Monica Moya, Irvine, CA (US); and Yu-Hsiang Hsu, Pasadena, CA (US)
Filed on Oct. 5, 2011, as Appl. No. 13/253,820.
Claims priority of provisional application 61/404,619, filed on Oct. 5, 2010.
Prior Publication US 2012/0083425 A1, Apr. 5, 2012
Int. Cl. G01N 33/50 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC G01N 33/5082 (2013.01) [C12N 5/0062 (2013.01); C12N 5/0691 (2013.01); G01N 33/5008 (2013.01); G01N 33/5011 (2013.01); C12N 2533/30 (2013.01); C12N 2535/00 (2013.01)] 25 Claims
 
1. A three-dimensional cell system, comprising:
a plurality of microfabricated microfluidic channels including a first microfabricated microfluidic channel and a second microfabricated microfluidic channel formed within a non-biological supportive material;
one or more microfabricated compartments formed within the non-biological supportive material;
cells combined with a biomimetic matrix and residing in at least one microfabricated compartment of the one or more microfabricated compartments; and
one or more living microvessels formed subsequent to placement of the cells combined with the biomimetic matrix within the at least one microfabricated compartment, wherein the one or more living microvessels including a first living microvessel that connects to the first microfabricated microfluidic channel and the second microfabricated microfluidic channel, the first living microvessel having a lumen and running from the first microfabricated microfluidic channel, through the at least one microfabricated compartment, and to the second microfabricated microfluidic channel, and the one or more living microvessels perfusing the cells combined with the biomimetic matrix and coupling together the plurality of microfabricated microfluidic channels,
wherein the one or more living microvessels allow for (i) delivery of nutrients from a fluid flowing from the first microfabricated microfluidic channel of the plurality of microfabricated microfluidic channels to the cells combined with the biomimetic matrix and (ii) removal of at least waste products from the fluid flowing to the second microfabricated microfluidic channel of the plurality of microfabricated microfluidic channels,
wherein the fluid flows inside the lumen of the one or more living microvessels, and wherein the one or more living microvessels are selected from a group consisting of (i) one or more living lymphatic vessels and (ii) one or more living blood vessels.