US 9,810,684 B2
Method for increasing number of stem cells in human or animal bodies
James Wang, Monterey Park, CA (US); Steve K Chen, Pacific Palisades, CA (US); Mou-Shiung Lin, Hsin-Chu (TW); and Yun Yen, Arcadin, CA (US)
Assigned to StemBios Technologies, Inc., Monterey Park, CA (US)
Filed by StemBios Technologies, Inc., Monterey Park, CA (US)
Filed on Dec. 19, 2013, as Appl. No. 14/135,553.
Claims priority of provisional application 61/740,507, filed on Dec. 21, 2012.
Claims priority of provisional application 61/819,529, filed on May 3, 2013.
Prior Publication US 2014/0178886 A1, Jun. 26, 2014
Int. Cl. G01N 33/53 (2006.01); G01N 33/50 (2006.01)
CPC G01N 33/5073 (2013.01) [G01N 33/5008 (2013.01); G01N 2333/70596 (2013.01)] 14 Claims
 
1. A method of determining an effect of one or more actions, comprising:
obtaining a first peripheral blood sample from a subject before the subject takes a first action;
performing a first purification process on said first blood sample, wherein said first purification process includes incubating said first blood sample with ethylenediaminetetraacetic acid (EDTA) and isolating from the thus incubated first blood sample a first population of small cells that are between 1.0 and 6.0 micrometers in size;
obtaining the number or percentage of CD349(+) somatic stem cells in the first population of small cells with flow cytometry;
obtaining a second peripheral blood sample from said subject after the subject takes the first action;
performing a second purification process on said second blood sample, wherein said second purification process includes incubating said second blood sample with EDTA and isolating from the thus incubated second blood sample a second population of small cells that are between 1.0 and 6.0 micrometers in size;
obtaining the number or percentage of CD349(+) somatic stem cells in the second population of small cells with flow cytometry; and
determining a change in the number or percentage of CD349(+) somatic stem cells in the second population as compared to the first population, whereby an effect of one or more actions is determined.