US 9,810,683 B2
Use of live cell inteferometry with reflective floor of observation chamber to determine changes in mass of mammalian cells
James K. Gimzewski, Topanga, CA (US); Jason C. Reed, Los Angeles, CA (US); and Michael A. Teitell, Tarzana, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Nov. 25, 2013, as Appl. No. 14/88,992.
Application 14/088,992 is a continuation of application No. 12/436,702, filed on May 6, 2009, granted, now 8,599,383.
Prior Publication US 2014/0080171 A1, Mar. 20, 2014
Int. Cl. G01B 9/02 (2006.01); G01N 33/50 (2006.01); G01J 3/453 (2006.01); G02B 5/126 (2006.01); G02B 21/00 (2006.01); G02B 21/32 (2006.01)
CPC G01N 33/5008 (2013.01) [G01J 3/453 (2013.01); G02B 5/126 (2013.01); G02B 21/0056 (2013.01); G02B 21/32 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for determining changes in optical thickness and/or mass of a mammalian cell, the method comprising:
(a) placing a mammalian cell in an observation chamber of a microscope, wherein the observation chamber is adapted to form an aqueous environment, and wherein said observation chamber comprises a reflective floor;
(b) using an interference microscope to detect changes in optical path length introduced by said cell as light passes through said cell and is reflected back from said reflective floor;
(c) altering the first environment to form a second environment;
(d) using said interference microscope to detect changes in optical path length introduced by said cell as light passes through said cell in said second environment and is reflected back from said reflective floor;
(e) comparing the observations in (b) with the observations in (d) so as to determine changes in the optical thickness and/or mass of said cell resulting from the alteration of said first environment to said second environment.