US 9,810,669 B2
Techniques for quantification of samples
Scott J. Geromanos, Middletown, NJ (US); Johannes P C Vissers, Huizen (NL); and James I. Langridge, Sale (GB)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Appl. No. 14/360,395
Filed by Waters Technologies Corporation, Milford, MA (US)
PCT Filed Nov. 16, 2012, PCT No. PCT/US2012/065441
§ 371(c)(1), (2) Date May 23, 2014,
PCT Pub. No. WO2013/081852, PCT Pub. Date Jun. 6, 2013.
Claims priority of provisional application 61/564,036, filed on Nov. 28, 2011.
Prior Publication US 2014/0330524 A1, Nov. 6, 2014
Int. Cl. G01N 33/48 (2006.01); G01N 30/72 (2006.01); H01J 49/00 (2006.01); G01N 33/68 (2006.01); G01N 33/92 (2006.01); G06G 7/58 (2006.01)
CPC G01N 30/7206 (2013.01) [G01N 33/68 (2013.01); G01N 33/92 (2013.01); H01J 49/0036 (2013.01); F04C 2270/041 (2013.01); G01N 30/7233 (2013.01)] 37 Claims
 
1. A method of performing protein quantification comprising:
receiving ionization intensities for precursor ions and product ions detected in an experiment that analyzes a sample including a known concentration or quantity of a predetermined protein, said precursor ions and said product ions originating from the predetermined protein in the sample, wherein the ionization intensities are generated from performing the experiment including performing liquid chromatography and mass spectrometry;
determining a first set of M precursor ions originating from the predetermined protein, wherein the first set of M precursor ions have the M highest ionization intensities of the precursor ions originating from the predetermined protein;
determining, for each of the M precursor ions in the first set, a second set of N product ions that are fragments obtained by fragmentation of said each precursor ion, wherein the second set of N product ions have the N highest ionization intensities of product ions that are fragments obtained by fragmentation of said each precursor ion;
calculating an intensity sum for the predetermined protein by adding ionization intensities of product ions included in the second sets for the M precursor ions in the first set;
determining, in accordance with the known concentration or quantity of the predetermined protein in the sample and the intensity sum, information included in a calibration standard;
performing a subsequent sample analysis using the calibration standard; and
determining, using the calibration standard and subsequent experimental data obtained from the subsequent sample analysis, a quantity of a protein in another sample analyzed in the subsequent sample analysis.