US 9,810,625 B2
Method for detecting and quantifying fibrosis
Vincent Vuiblet, Chenay (FR); Olivier Piot, Cormontreuil (FR); Philippe Rieu, Merfy (FR); Michael Fere, Reims (FR); and Cyril Gobinet, Athies-Sous-Laon (FR)
Assigned to UNIVERSITE DE REIMS CHAMPAGNE-ARDENNE, Reims (FR)
Appl. No. 15/329,011
Filed by UNIVERSITE DE REIMS CHAMPAGNE-ARDENNE, Reims (FR)
PCT Filed Jul. 2, 2015, PCT No. PCT/FR2015/051826
§ 371(c)(1), (2) Date Jan. 25, 2017,
PCT Pub. No. WO2016/016531, PCT Pub. Date Feb. 4, 2016.
Claims priority of application No. 14 57323 (FR), filed on Jul. 29, 2014.
Prior Publication US 2017/0212041 A1, Jul. 27, 2017
Int. Cl. G01N 21/35 (2014.01); G01N 21/3563 (2014.01); G01N 33/50 (2006.01); G01N 21/31 (2006.01)
CPC G01N 21/3563 (2013.01) [G01N 33/5091 (2013.01); G01N 2021/3196 (2013.01); G01N 2021/3595 (2013.01); G01N 2201/10 (2013.01); G01N 2201/129 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for detecting and quantifying fibrosis, the method comprising the steps of:
positioning a fixed non-colored tissue sample on a support;
performing an infrared acquisition by sweeping points of said sample;
producing an infrared spectrum, wherein each acquired point is converted into at least one spectral image;
digital processing of the spectral image is carried out by discrimination between at least physiological collagen and pathological collagen from fibrosis by assigning to each point a spectral class defined by an algorithm classifying the spectra according to spectral similarities;
assigning a condition to each class by statistical comparison with a numerical model of spectra;
quantifying a relative surface area occupied by the points of each condition; and
recording a result.