US 9,810,610 B2
Method of partial lysis and assay
Anthony P. Shuber, Northbridge, MA (US); Steven Hecht, Boston, MA (US); Erin Coffman, Nashua, NH (US); Paul MacLean, Marlborough, MA (US); and Howard B. Kaufman, Newton, MA (US)
Assigned to Hologic, Inc., Marlborough, MA (US)
Filed by Hologic, Inc., Bedford, MA (US)
Filed on Sep. 17, 2015, as Appl. No. 14/856,611.
Claims priority of provisional application 62/051,672, filed on Sep. 17, 2014.
Prior Publication US 2016/0076980 A1, Mar. 17, 2016
Int. Cl. G01N 1/30 (2006.01)
CPC G01N 1/30 (2013.01) 26 Claims
OG exemplary drawing
 
1. A method of evaluating a biological sample comprising cells, said method comprising:
obtaining a biological sample from a subject;
contacting the biological sample with a biological sample preservative to create a preserved biological sample;
partially lysing the preserved biological sample to create a mixture comprising lysed cells, whole cells, and molecular components;
characterizing a first portion of the mixture using cell based analysis, wherein cell based analysis comprises mounting whole cells on a slide; and
assaying a second portion of the mixture for molecular components.