US 9,809,904 B2
Methods for retrieval of sequence-verified DNA constructs
Jay Shendure, Seattle, WA (US); Jerrod Schwartz, Seattle, WA (US); Jacob Kitzman, Seattle, WA (US); Rupali Patwardhan, Seattle, WA (US); and Joseph Hiatt, Seattle, WA (US)
Assigned to UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION, Seattle, WA (US)
Filed by Jay Shendure, Seattle, WA (US); Jerrod Schwartz, Seattle, WA (US); Jacob Kitzman, Seattle, WA (US); Rupali Patwardhan, Seattle, WA (US); and Joseph Hiatt, Seattle, WA (US)
Filed on Apr. 23, 2012, as Appl. No. 13/453,947.
Claims priority of provisional application 61/478,016, filed on Apr. 21, 2011.
Prior Publication US 2012/0283110 A1, Nov. 8, 2012
Int. Cl. C40B 20/04 (2006.01); C12Q 1/68 (2006.01)
CPC C40B 20/04 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6853 (2013.01)] 16 Claims
 
1. A method of recovering a sequence-verified target nucleic acid from a sequenced nucleic acid library comprising
tagging each member of a nucleic acid library with at least one identification tag having a degenerate sequence;
sequencing the tagged members of the nucleic acid library; and
selectively recovering only the sequence-verified target nucleic acid from the tagged and sequenced members of the nucleic acid library by targeting the at least one identification tag that is unique to the sequence-verified target nucleic acid using a clone-free dial-out selection method and amplifying the sequence-verified target nucleic acids.