US 9,809,862 B2
G-protein coupled receptor (GPCR)-based biosensors and uses thereof
Pamela Peralta-Yahya, Atlanta, GA (US); Kuntal Mukherjee, Atlanta, GA (US); Souryadeep Bhattacharyya, Atlanta, GA (US); and Stephen Sarria, Atlanta, GA (US)
Assigned to Georgia Tech Research Corporation, Atlanta, GA (US)
Filed by Georgia Tech Research Corporation, Atlanta, GA (US)
Filed on Aug. 11, 2015, as Appl. No. 14/823,317.
Claims priority of provisional application 62/035,734, filed on Aug. 11, 2014.
Prior Publication US 2016/0122832 A1, May 5, 2016
Int. Cl. C12Q 1/68 (2006.01); G01N 33/92 (2006.01); C12N 15/81 (2006.01); G01N 33/58 (2006.01); G01N 33/74 (2006.01)
CPC C12Q 1/6897 (2013.01) [C12N 15/81 (2013.01); G01N 33/582 (2013.01); G01N 33/74 (2013.01); G01N 33/92 (2013.01); G01N 2333/726 (2013.01)] 20 Claims
 
1. An engineered yeast cell comprising:
a sensing unit, the sensing unit comprising:
a G-protein coupled receptor (GPCR);
a synthetic processing unit, the synthetic processing unit comprising:
a signal transduction pathway, wherein the signal transduction pathway comprises a synthetic transcription factor selected from the group consisting of STF1, STF2, SFT3 and combinations thereof; and
a synthetic response unit, the synthetic response unit comprising:
a recombinant signal molecule gene, where the recombinant signal molecule gene is operatively coupled to a synthetic promoter that is responsive to the synthetic processing unit, wherein the synthetic promoter is selected from the group consisting of: pGAL45x, pLExA4x, pCRE, and combinations thereof,
wherein the sensing unit is in biologic communication with the processing unit and the processing unit is in biologic communication with the response unit.