US 9,809,860 B2
Test kits and methods for their use in detecting expression ratios of genetic markers
Parry John Guilford, Dunedin (NZ)
Assigned to Pacific Edge Limited, Dunedin (NZ)
Filed by Pacific Edge Limited, Dunedin (NZ)
Filed on Oct. 22, 2015, as Appl. No. 14/920,552.
Application 14/920,552 is a division of application No. 12/843,435, filed on Jul. 26, 2010, granted, now 9,702,009.
Application 12/843,435 is a continuation of application No. 12/221,626, filed on Aug. 5, 2008, abandoned.
Prior Publication US 2016/0102368 A1, Apr. 14, 2016
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); C12Q 1/68 (2006.01); G01N 33/574 (2006.01); G06F 19/00 (2011.01)
CPC C12Q 1/6886 (2013.01) [G01N 33/57407 (2013.01); G06F 19/345 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)] 18 Claims
 
1. A test kit for detection of expression ratios of genetic markers for transitional cell carcinoma (TCC), comprising:
a forward polymerase chain reaction (PCR) primer consisting of the sequence of SEQ ID NO. 1 and a reverse PCR primer consisting of the sequence of SEQ ID NO. 2 for hybridizing to an mRNA or Cy3 or Cy5 labeled cDNA transcript of insulin-like growth factor binding protein 5 (“IGFBP5”);
a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe consisting of the sequence of SEQ ID NO. 3 that hybridizes to an mRNA or a cDNA transcript of said IGFBP5 between the binding locations of said forward and reverse primers of IGFBP5;
a forward PCR primer and a reverse PCR primer for hybridizing to an mRNA or a cDNA oligonucleotide transcript oligonucleotide of homeobox A13 (“HOXA13”);
a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe that hybridizes to an mRNA or a Cy3 or Cy5 Labeled cDNA transcript of said HOXA13 between the binding locations of said forward and reverse primers of HOXA13;
a forward PCR primer and a reverse PCR primer for hybridizing to an mRNA or cDNA transcript of midkine (neurite growth promoting factor 2 (“MDK”);
a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe that hybridizes to an mRNA or a Cy3 or Cy5 labeled cDNA transcript of said MDK between the binding locations of said forward and reverse primers of MDK;
a forward PCR primer and a reverse PCR primer for hybridizing to an mRNA or Cy3 or Cy5 labeled cDNA transcript of leukotriene B4 12-dehydrogenase (“LTB4DH”); and
a reporter fluorescent dye and a quencher fluorescent dye labeled PCR probe that hybridizes to an mRNA or a Cy3 or Cy5 labeled cDNA transcript of said LTB4DH between the binding locations of said forward and reverse primers of LTB4DH.