US 9,809,857 B2
Methods and signatures for oropharyngeal cancer prognosis
Xiaowei Wang, St. Louis, MO (US)
Filed by The Washington University, St. Louis, MO (US)
Filed on Feb. 28, 2014, as Appl. No. 14/193,975.
Claims priority of provisional application 61/770,730, filed on Feb. 28, 2013.
Prior Publication US 2014/0243219 A1, Aug. 28, 2014
Int. Cl. C12Q 1/68 (2006.01)
CPC C12Q 1/6886 (2013.01) [C12Q 2600/112 (2013.01)] 18 Claims
1. A method of identifying a subject with oropharyngeal cancer as having a poor or a favorable prognosis, the method comprising:
(a) obtaining a tissue sample yielding low quality RNA from a subject;
(b) using a PCR-based profiling method for miRNA expression, to determine in the sample, the levels of six prognostic miRNAs selected from the group consisting of miR-142-3p, miR-31, miR-146a, miR-26b, miR-24, and miR-193b;
(c) calculating a risk score using the levels of miRNAs determined, and
(d) comparing the risk score to a median risk score,
wherein, if (i) the risk score is equal to or higher than the median risk score, the subject has a poor prognosis, and (ii) the risk score is lower than the median risk score, the subject has a favorable prognosis, wherein poor prognosis is indicative of a significantly greater risk of death.