US 9,809,852 B2
Systems and methods for biological analysis
Hesaam Esfandyarpour, Redwood City, CA (US); Hamid Rategh, Cupertino, CA (US); Meysam R. Barmi, Palo Alto, CA (US); Kosar B. Parizi, Redwood City, CA (US); and Kambiz Kaviani, Palo Alto, CA (US)
Assigned to GENAPSYS, INC., Redwood City, CA (US)
Appl. No. 14/653,230
Filed by GENAPSYS, INC., Redwood City, CA (US)
PCT Filed Mar. 14, 2014, PCT No. PCT/US2014/027544
§ 371(c)(1), (2) Date Jun. 17, 2015,
PCT Pub. No. WO2014/152625, PCT Pub. Date Sep. 25, 2014.
Claims priority of provisional application 61/799,396, filed on Mar. 15, 2013.
Claims priority of provisional application 61/799,483, filed on Mar. 15, 2013.
Claims priority of provisional application 61/799,944, filed on Mar. 15, 2013.
Claims priority of provisional application 61/800,410, filed on Mar. 15, 2013.
Claims priority of provisional application 61/800,443, filed on Mar. 15, 2013.
Claims priority of provisional application 61/801,560, filed on Mar. 15, 2013.
Claims priority of provisional application 61/801,929, filed on Mar. 15, 2013.
Prior Publication US 2015/0376692 A1, Dec. 31, 2015
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2006.01); G01N 27/74 (2006.01)
CPC C12Q 1/6874 (2013.01) [G01N 27/745 (2013.01)] 12 Claims
 
1. A method for nucleic acid sequencing, comprising:
(a) directing a plurality of particles onto an array of sensors, wherein an individual particle among said plurality of particles comprises a template nucleic acid molecule coupled thereto, wherein said array comprises a plurality of sensors, wherein an individual sensor among said plurality of sensors comprises a transmitter electrode and a receiver electrode, which transmitter electrode or receiver electrode is coupled to at least another individual sensor among said plurality of sensors;
(b) positioning said individual particle adjacent to said individual sensor;
(c) performing a primer extension reaction on said template nucleic acid molecule coupled to said individual particle at said individual sensor; and
(d) during or subsequent to performing said primer extension reaction, measuring a signal that is indicative of a change in impedance between said transmitter electrode and receiver electrode.