US 9,809,849 B2
Methods and systems for pure dye instrument normalization
Jeffrey Marks, Mountain View, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed on Feb. 5, 2016, as Appl. No. 15/16,713.
Claims priority of provisional application 62/112,964, filed on Feb. 6, 2015.
Prior Publication US 2016/0237474 A1, Aug. 18, 2016
Int. Cl. G01N 21/64 (2006.01); C12Q 1/68 (2006.01); G01N 21/27 (2006.01)
CPC C12Q 1/686 (2013.01) [G01N 21/274 (2013.01); G01N 21/6452 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6421 (2013.01); G01N 2201/04 (2013.01); G01N 2201/068 (2013.01); G01N 2201/12746 (2013.01)] 16 Claims
OG exemplary drawing
1. A method for normalizing laboratory instruments with pure dyes, comprising:
providing at least one reference instrument and a test instrument, each instrument comprising at least one excitation filter and at least one emission filter arranged in pairs;
providing a plurality of pure dyes, each dye comprising a fluorescent component and contained in a pure dye plate comprising a plurality of wells;
generating fluorescent spectra from the reference instrument and the test instrument for multiple pure dyes across multiple filter combinations;
creating a pure dye matrix, Mref, for the reference instrument and a pure dye matrix, M, for the test instrument;
calculating correction factors for each filter pair and multiplying the correction factors by the pure dye spectra;
normalizing the corrected pure dye spectra;
generating multicomponent data.