US 9,809,846 B2
Compositions, kits, uses and methods for amplified detection of an analyte
Itamar Willner, Mevasseret Zion (IL); Fuan Wang, Wuhan (CN); Chun-Hua Lu, Fujian (CN); Xiaoqing Liu, Wuhan (CN); and Lina Freage, Kfar Kassem (IL)
Assigned to Yissum Research Development Company of The Hebrew University Of Jerusalem Ltd., Jerusalem (IL)
Filed by YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM Ltd., Jerusalem (IL)
Filed on Dec. 30, 2014, as Appl. No. 14/586,214.
Claims priority of provisional application 61/922,076, filed on Dec. 30, 2013.
Claims priority of provisional application 61/923,408, filed on Jan. 3, 2014.
Prior Publication US 2015/0197804 A1, Jul. 16, 2015
Int. Cl. C12Q 1/68 (2006.01); C07H 21/04 (2006.01)
CPC C12Q 1/6844 (2013.01) [C12Q 1/6816 (2013.01)] 20 Claims
 
1. A composition comprising:
at least one hairpin selected from the group consisting of SEQ. ID. NO. 4 and SEQ. ID. NO. 8, the at least one hairpin sequence comprising
at least one analyte sequence I′, and
at least one sequence II; and
at least one circular sequence selected from the group consisting of SEQ. ID. NO. 2, SEQ. ID. NO. 6 and SEQ. ID. NO. 9, the at least one circular sequence comprising
at least one sequence I being complementary with sequence I′,
at least one sequence II, and
at least one sequence III encoding a biosensor sequence III′.