US 9,809,844 B2
Method of DNA analysis using micro/nanochannel
David C. Schwartz, Madison, WI (US); Kyubong Jo, Champaign, IL (US); and Dalia M. Dhingra, Arlington Heights, IL (US)
Assigned to Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed by David C. Schwartz, Madison, WI (US); Kyubong Jo, Champaign, IL (US); and Dalia M. Dhingra, Arlington Heights, IL (US)
Filed on May 13, 2011, as Appl. No. 13/107,400.
Application 13/107,400 is a continuation of application No. 11/633,232, filed on Nov. 29, 2006, granted, now 7,960,105.
Claims priority of provisional application 60/740,583, filed on Nov. 29, 2005.
Claims priority of provisional application 60/740,693, filed on Nov. 30, 2005.
Prior Publication US 2011/0275066 A1, Nov. 10, 2011
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); C12Q 1/68 (2006.01); B82Y 15/00 (2011.01); B82Y 30/00 (2011.01)
CPC C12Q 1/6837 (2013.01) [B82Y 15/00 (2013.01); B82Y 30/00 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6869 (2013.01); B01L 3/5027 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method of preparing a double-stranded DNA molecule for optical analysis, the method comprising the steps of:
ligating the DNA molecule to remove inherent nicks;
nicking at least one predefined sequence position of one strand of the DNA molecule without breaking the double-stranded DNA molecule into wholly separate pieces, wherein at least one nicked position defines a pattern-characteristic of the nucleotide sequence of the DNA molecule;
elongating the DNA molecule in a buffer having an ionic strength sufficiently low to increase the persistence length of the DNA molecule;
labeling the DNA molecule with at least one fluorescent label wherein the fluorescent label is specific to the nicked position; and
introducing the elongated and labeled DNA molecule suspended in the buffer into a channel to maintain the elongated and labeled DNA molecule in an elongated state capable of being analyzed optically without fixation to the channel.