US 9,809,842 B2
Chemiluminescence compact imaging scanner
Ahmed Bouzid, Lincoln, NE (US); Chris Lesiak, Lincoln, NE (US); Daniel W. Konz, Lincoln, NE (US); David L. Franzen, Lincoln, NE (US); William Biggs, Lincoln, NE (US); and Jon P. Anderson, Lincoln, NE (US)
Assigned to LI-COR, Inc., Lincoln, NE (US)
Filed by LI-COR, Inc., Lincoln, NE (US)
Filed on Mar. 24, 2014, as Appl. No. 14/223,672.
Application 14/223,672 is a division of application No. 13/919,877, filed on Jun. 17, 2013, granted, now 8,722,346.
Application 13/919,877 is a continuation of application No. 13/432,410, filed on Mar. 28, 2012, granted, now 8,486,644, issued on Jul. 16, 2013.
Claims priority of provisional application 61/576,941, filed on Dec. 16, 2011.
Prior Publication US 2014/0206568 A1, Jul. 24, 2014
Int. Cl. C12Q 1/68 (2006.01); G01N 21/76 (2006.01); H04N 1/10 (2006.01); H04N 1/193 (2006.01); G01N 21/64 (2006.01); G01J 1/04 (2006.01); G01N 33/53 (2006.01); G01N 21/62 (2006.01); G01N 33/543 (2006.01); H04N 1/191 (2006.01)
CPC C12Q 1/6825 (2013.01) [G01J 1/0403 (2013.01); G01N 21/62 (2013.01); G01N 21/6486 (2013.01); G01N 21/76 (2013.01); G01N 33/53 (2013.01); G01N 33/5302 (2013.01); G01N 33/54373 (2013.01); H04N 1/1017 (2013.01); H04N 1/191 (2013.01); H04N 1/1933 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of scanning a luminescent sample, the method comprising:
providing a detector bar having one or more image sensor arrays, each image sensor array having a plurality of pixel elements;
placing on a sample bed a luminescent sample having luminescence;
moving the detector bar with respect to the sample bed in a first pass;
taking a first set of pixel readings over predetermined exposure times during the first pass, each pixel reading of the first set occurring at a respective x, y position;
moving the detector bar with respect to the sample bed in a second pass, the second pass being in an opposite direction from the first pass;
collecting a second set of pixel readings over predetermined exposure times during the second pass, wherein the first and second passes are conducted while the luminescent sample constantly fades in luminescence over time; and
summing respective position x, y pixel readings between the first and second sets of pixel readings over the respective predetermined exposure times to construct an image, the summing of the first and second sets of pixel readings compensating for the constantly fading luminescence of the luminescent sample.