US 9,809,841 B2
Detection of nucleic acid sequence differences by comparative genomic hybridization
Donna G. Albertson, New York, NY (US); Daniel Pinkel, New York, NY (US); Yevgeniya Fridlyand, San Francisco, CA (US); Bing Huey, San Francisco, CA (US); and Antoine Snijders, Antioch, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Apr. 20, 2016, as Appl. No. 15/134,331.
Application 15/134,331 is a continuation of application No. 12/405,867, filed on Mar. 17, 2009, abandoned.
Application 12/405,867 is a continuation of application No. 11/060,644, filed on Feb. 16, 2005, granted, now 7,534,567, issued on May 19, 2009.
Claims priority of provisional application 60/545,429, filed on Feb. 17, 2004.
Prior Publication US 2016/0340718 A1, Nov. 24, 2016
Int. Cl. C12Q 1/68 (2006.01)
CPC C12Q 1/6809 (2013.01) 33 Claims
 
1. An array Comparative Genomic Hybridization (CGH) method of detecting one or more nucleotide sequence differences in nucleic acid sequences in a first sample relative to nucleic acid sequences in a second sample, said method comprising:
(a) labeling nucleic acids from each sample with a different label;
(b) contacting the labeled nucleic acids from each sample with target nucleic acids, wherein either the labeled nucleic acids or the target nucleic acids, or both, have had repetitive sequences, if initially present, blocked and/or removed; and
(c) comparing the intensities of the signals from labeled nucleic acids hybridized to the target nucleic acids to detect one or more nucleotide sequence differences between the samples, wherein:
said contacting comprises contacting the labeled nucleic acids from each sample with an array of target elements comprising the target nucleic acids;
said comparing comprises comparing the intensities of the signals from labeled nucleic acids hybridized to each target element; and
the sequence complexity of each target element is greater than about 50 kilobases and the sequence divergence between the samples is less than about 10%.