US 9,809,838 B2
Methods for determining the concentration of an analyte in solution
David R. Walt, Boston, MA (US); and David M. Rissin, Somerville, MA (US)
Assigned to Trustees of Tufts College, Medford, MA (US)
Filed by Trustees of Tufts College, Medford, MA (US)
Filed on Oct. 11, 2016, as Appl. No. 15/290,939.
Application 15/290,939 is a continuation of application No. 12/675,686, abandoned, previously published as PCT/US2007/019184, filed on Aug. 30, 2007.
Prior Publication US 2017/0159104 A1, Jun. 8, 2017
Int. Cl. C12Q 1/00 (2006.01); C12Q 1/40 (2006.01); G01N 33/68 (2006.01)
CPC C12Q 1/40 (2013.01) [G01N 33/6854 (2013.01); G01N 2333/522 (2013.01); G01N 2333/938 (2013.01)] 10 Claims
 
1. A method of determining the concentration of an analyte in a fluid sample to be tested, the method comprising the steps of:
(a) partitioning at least a portion of the analyte molecules in the fluid sample across a plurality of reaction vessels so that more than 20% but less than 95% of the reaction vessels contain at least one molecule, wherein the analyte is attached to a nanoparticle or microparticle by a capture component;
(b) determining the presence or absence of the analyte in each reaction vessel to identify the number of reaction vessels that contain analyte and/or to identify the number of reaction vessels that contain no analyte; and
(c) determining the concentration of the analyte in the fluid sample by a Gaussian distribution analysis of the number of reaction vessels that contain the analyte.