US 9,809,834 B2
Processes of treating cellulosic material
Xin Li, Raleigh, NC (US); Mads Torry Smith, Raleigh, NC (US); Brandon Emme, Kansas City, MO (US); and Lorraine Putnam, Youngsville, NC (US)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Appl. No. 14/402,897
Filed by NOVOZYMES A/S, Bagsvaerd (DK); and NOVOZYMES NORTH AMERICA, INC., Franklinton, NC (US)
PCT Filed May 29, 2013, PCT No. PCT/US2013/043084
§ 371(c)(1), (2) Date Nov. 21, 2014,
PCT Pub. No. WO2013/181233, PCT Pub. Date Dec. 5, 2013.
Claims priority of provisional application 61/652,559, filed on May 29, 2012.
Prior Publication US 2015/0176043 A1, Jun. 25, 2015
Int. Cl. C12P 19/14 (2006.01); C12P 19/02 (2006.01); C13K 1/02 (2006.01); C13K 13/00 (2006.01)
CPC C12P 19/14 (2013.01) [C12P 19/02 (2013.01); C12Y 110/03002 (2013.01); C13K 1/02 (2013.01); C13K 13/002 (2013.01); C12P 2201/00 (2013.01); C12P 2203/00 (2013.01)] 17 Claims
 
1. A process of producing a fermentation product from pretreated cellulosic material, comprising the steps of:
i) preconditioning said pretreated cellulosic material;
ii) hydrolyzing said cellulosic material of step i) in the presence of a cellulolytic enzyme preparation to produce sugars;
iii) fermenting said sugars of step ii) in the presence of a microorganism to produce said fermentation product;
wherein a solid-liquid separation step, resulting in a solid fraction and a liquid fraction, is carried out:
a) after preconditioning step i), but before hydrolyzing step ii); or
b) after hydrolyzing step ii), but before fermenting step iii);
wherein a phenol oxidizing enzyme is present or added:
during preconditioning step i); or
after preconditioning step i), but before hydrolyzing step ii); or
during hydrolyzing step ii);
wherein a hemicellulase is present or added:
during preconditioning step i); or
after preconditioning step i), but before hydrolyzing step ii); or
during hydrolyzing step ii)
wherein said solid-liquid separation step is carried out after said cellulosic material is subjected to said phenol oxidizing enzyme and said hemicellulase; and wherein separation into the solid fraction and the liquid fraction is improved as compared to the same process where said phenol oxidizing enzyme and said hemicellulase are not present or added.