US 9,809,833 B2
Pathways to adipate semialdehyde and other organic products
Man Kit Lau, Plymouth, MN (US); and Christopher P. Mercogliano, Minneapolis, MN (US)
Assigned to BioAmber Inc., St. Paul, MN (US)
Appl. No. 14/418,543
Filed by BioAmber Inc., Plymouth, MN (US)
PCT Filed Sep. 20, 2013, PCT No. PCT/US2013/060853
§ 371(c)(1), (2) Date Jan. 30, 2015,
PCT Pub. No. WO2014/047407, PCT Pub. Date Mar. 27, 2014.
Claims priority of provisional application 61/703,361, filed on Sep. 20, 2012.
Prior Publication US 2015/0291987 A1, Oct. 15, 2015
Int. Cl. C12P 13/00 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/16 (2006.01); C12N 9/88 (2006.01); C12P 7/18 (2006.01); C12P 7/40 (2006.01); C12P 7/44 (2006.01)
CPC C12P 13/001 (2013.01) [C12N 9/001 (2013.01); C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/1096 (2013.01); C12N 9/13 (2013.01); C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12P 7/18 (2013.01); C12P 7/40 (2013.01); C12P 7/44 (2013.01); C12P 13/005 (2013.01); C12Y 101/01 (2013.01); C12Y 103/01 (2013.01); C12Y 103/08 (2013.01); C12Y 206/01 (2013.01); C12Y 208/03 (2013.01); C12Y 301/02 (2013.01); C12Y 402/01 (2013.01)] 11 Claims
 
1. A recombinant microorganism genetically engineered to produce adipate semialdehyde from 4-hydroxybutyryl-CoA, the recombinant microorganism comprising a first exogenous nucleic acid encoding a CoA synthase of class E.C. 2.3.1.X that catalyzes a substrate to product conversion of malonyl-CoA and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA and CO2, wherein the recombinant microorganism produces adipate semialdehyde from 4-hydroxybutyryl-CoA via the intermediates: 6-hydroxy-3-oxo-hexanoyl-CoA; 3,6-dihydroxyhexanoyl-CoA; 6-hydroxy-2-hexenoyl-CoA; 6-hydroxyhexanoyl-CoA; and 6-hydroxycaproic acid.