US 9,809,832 B2
Microbial biotransformation of aromatic acids to their reduced carbon aromatic acids
Mahendra M Yeole, Mumbai (IN); Aparna Gala, Mumbai (IN); and Arvind M. Lali, Mumbai (IN)
Assigned to PRIVI BIOTECHNOLOGIES PRIVATE LIMITED, Navi Mumbai, Maharashtra (IN)
Appl. No. 14/424,634
PCT Filed Aug. 28, 2013, PCT No. PCT/IN2013/000525
§ 371(c)(1), (2) Date Feb. 27, 2015,
PCT Pub. No. WO2014/045298, PCT Pub. Date Mar. 27, 2014.
Claims priority of application No. 2496/MUM/2012 (IN), filed on Aug. 28, 2012.
Prior Publication US 2015/0211029 A1, Jul. 30, 2015
Int. Cl. C12P 7/40 (2006.01); C12P 7/42 (2006.01)
CPC C12P 7/42 (2013.01) 21 Claims
1. A process for a biotransformation of aromatic acids to their reduced carbon aromatic acids comprising:
a) culturing or growing Amycolatopsis mediterranei NCIM 5008 in a fermentor at a temperature in a range of 37-46° C. for 16-24 hours;
b) adding an alkaline solution of substrate containing aromatic acid to a fermentation broth of step (a) to obtain the final concentration of 1-15 g/L for aromatic acid; wherein aromatic acid is converted for 24-50 hours and converted liquid containing reduced carbon aromatic acid is obtained;
c) centrifuging or filtrating the converted liquid of step (b) to obtain retentate comprising culture cells, and permeate comprising converted liquid with reduced carbon aromatic acid;
d) precipitating the converted liquid obtained from step (c) to isolate crude reduced carbon aromatic acid in solid form; and
e) recrystallizing the crude reduced carbon aromatic acid from (d) to obtain pure reduced carbon aromatic acid.