US 9,809,830 B2
Mutant NNK1 allele and its use
Johan Thevelein, Blanden (BE); Mekonnen Demeke, Leuven (BE); and Maria Remedios Foulquié Moreno, Brussels (BE)
Assigned to VIB VZW, Ghent (BE); and KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&D, Leuven (BE)
Appl. No. 15/103,916
Filed by VIB VZW, Ghent (BE); and KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&D, Leuven (BE)
PCT Filed Dec. 12, 2014, PCT No. PCT/EP2014/077528
§ 371(c)(1), (2) Date Jun. 13, 2016,
PCT Pub. No. WO2015/086805, PCT Pub. Date Jun. 18, 2015.
Claims priority of application No. 13197206 (EP), filed on Dec. 13, 2013.
Prior Publication US 2016/0312244 A1, Oct. 27, 2016
Int. Cl. C12P 7/06 (2006.01); C12N 1/14 (2006.01); C12N 9/12 (2006.01); C12P 7/10 (2006.01)
CPC C12P 7/06 (2013.01) [C12N 9/12 (2013.01); C12P 7/10 (2013.01); C12Y 207/11001 (2013.01); Y02E 50/16 (2013.01); Y02E 50/17 (2013.01)] 18 Claims
 
1. A genetically modified xylose fermenting yeast strain, comprising a mutant DNA molecule that corresponds to a mutant nitrogen network kinase 1 (NNK1) allele and encodes a mutant kinase protein carrying a mutation at position 807 of a wild type kinase protein the amino acid sequence of which is 95% identical to SEQ ID NO: 2, wherein the yeast strain is not Saccharomyces cerevisiae strain GS1.11-26 and wherein the mutant increases the xylose-to-ethanol fermentation rate as compared to the wild type.