US 9,809,825 B2
Method for producing archaeal protein
Kazuhiko Ishikawa, Osaka (JP); Seiichirou Kishishita, Hiroshima (JP); and Tatsuya Fujii, Hiroshima (JP)
Assigned to National Institute of Advanced Industrial Science and Technology, Tokyo (JP)
Filed by National Institute of Advanced Industrial Science and Technology, Tokyo (JP)
Filed on Oct. 7, 2016, as Appl. No. 15/288,203.
Application 15/288,203 is a continuation in part of application No. PCT/JP2015/061068, filed on Apr. 9, 2015.
Claims priority of application No. 2014-081115 (JP), filed on Apr. 10, 2014.
Prior Publication US 2017/0159062 A1, Jun. 8, 2017
Int. Cl. C12N 9/24 (2006.01); C12N 15/80 (2006.01); C12N 9/42 (2006.01)
CPC C12N 15/80 (2013.01) [C12N 9/2437 (2013.01); C12Y 302/01004 (2013.01)] 16 Claims
 
1. A method of producing an archaeal protein, comprising a step of cultivating a fungus belonging to the genus Talaromyces,
wherein the fungus has been transformed with an expression vector comprising:
a promoter region that functions in the fungus;
a secretion signal region located downstream of the promoter region, the secretion signal region encoding a secretion signal peptide of a cellobiohydrolase gene from the genus Talaromyces;
a coding region for the archaeal protein located downstream of the secretion signal region; and
a terminator region located downstream of the coding region, and
wherein the fungus secretes the archaeal protein.