US 9,809,811 B2
Method of random circular permutation by MuCP-ISC and MuCP-ISSC transposons
Jin Ryoun Kim, Jericho, NY (US); Brennal Pierre, Brooklyn, NY (US); and Vandan Shah, Jersey City, NJ (US)
Assigned to New York University, New York, NY (US)
Filed by New York University, New York, NY (US)
Filed on Apr. 14, 2015, as Appl. No. 14/686,002.
Claims priority of provisional application 61/979,894, filed on Apr. 15, 2014.
Prior Publication US 2016/0083763 A1, Mar. 24, 2016
Int. Cl. C12N 15/10 (2006.01)
CPC C12N 15/102 (2013.01) 27 Claims
16. A method for designing circular permuted proteins, comprising:
a) constructing random circular permuted proteins using a Mu Circular permutation transposon (MuCP);
b) restricting the length of the appended amino acids to 1 at N-terminus for the MuCP and to 2 or 3 at C-terminus for the MuCP;
c) comprising giving flexibility to the length of the linker sequence connecting the N and C termini for the MuCP.