US 9,809,806 B2
Alpha-amylases
Carsten Andersen, Bagsvaerd (DK); and Thomas Agersten Poulsen, Bagsvaerd (DK)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Oct. 24, 2016, as Appl. No. 15/332,043.
Application 15/332,043 is a division of application No. 14/696,979, filed on Apr. 27, 2015, granted, now 9,506,047.
Application 14/696,979 is a division of application No. 14/064,911, filed on Oct. 28, 2013, granted, now 9,139,822, issued on Sep. 22, 2015.
Application 14/064,911 is a division of application No. 13/857,431, filed on Apr. 5, 2013, granted, now 8,591,969, issued on Nov. 26, 2013.
Application 13/857,431 is a division of application No. 13/514,727, granted, now 8,435,577, issued on May 7, 2013, previously published as PCT/EP2011/050073, filed on Jan. 4, 2011.
Claims priority of provisional application 61/362,536, filed on Jul. 8, 2010.
Claims priority of provisional application 61/355,230, filed on Jun. 16, 2010.
Claims priority of provisional application 61/354,817, filed on Jun. 15, 2010.
Claims priority of provisional application 61/354,775, filed on Jun. 15, 2010.
Claims priority of provisional application 61/333,930, filed on May 12, 2010.
Claims priority of provisional application 61/304,092, filed on Feb. 12, 2010.
Claims priority of provisional application 61/292,327, filed on Jan. 5, 2010.
Claims priority of provisional application 61/292,324, filed on Jan. 5, 2010.
Claims priority of application No. 10150062 (EP), filed on Jan. 4, 2010; and application No. 10150063 (EP), filed on Jan. 4, 2010.
Prior Publication US 2017/0037388 A1, Feb. 9, 2017
Int. Cl. C12N 9/24 (2006.01); C12N 9/28 (2006.01); C11D 3/386 (2006.01); C12P 7/06 (2006.01); C12P 19/04 (2006.01); C12P 19/14 (2006.01); C12N 9/26 (2006.01); C12P 7/14 (2006.01); C12P 19/02 (2006.01); C12N 15/82 (2006.01)
CPC C12N 9/2417 (2013.01) [C11D 3/386 (2013.01); C11D 3/38681 (2013.01); C12N 9/2414 (2013.01); C12N 15/8257 (2013.01); C12P 7/06 (2013.01); C12P 7/14 (2013.01); C12P 19/02 (2013.01); C12P 19/04 (2013.01); C12P 19/14 (2013.01); C12Y 302/01001 (2013.01); C12P 2203/00 (2013.01); Y02E 50/17 (2013.01)] 14 Claims
 
1. An isolated alpha-amylase comprising the A- and C-domains of a calcium-sensitive alpha-amylase and the B-domain of a calcium-insensitive alpha-amylase, wherein the A- and C-domains have at least 90% sequence identity with the A- and C-domains of SEQ ID NO: 5, and the B-domain has at least 90% sequence identity with the B-domain of SEQ ID NO: 13.