US 9,809,799 B2
Methods for inactivating viruses during a protein purification process
Alex Xenopoulos, Billerica, MA (US)
Assigned to EMD Millipore Corporation, Burlington, MA (US)
Appl. No. 14/390,924
Filed by EMD Millipore Corporation, Billerica, MA (US)
PCT Filed Jun. 13, 2013, PCT No. PCT/US2013/045677
§ 371(c)(1), (2) Date Oct. 6, 2014,
PCT Pub. No. WO2014/004103, PCT Pub. Date Jan. 3, 2014.
Claims priority of provisional application 61/666,145, filed on Jun. 29, 2012.
Prior Publication US 2015/0064769 A1, Mar. 5, 2015
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 7/00 (2006.01); C07K 1/16 (2006.01); C07K 1/36 (2006.01)
CPC C12N 7/00 (2013.01) [C07K 1/36 (2013.01); C07K 1/16 (2013.01); C12N 2740/10061 (2013.01)] 10 Claims
OG exemplary drawing
1. A method of inactivating one or more viruses in a Protein A eluate as the Protein A eluate flows from a Protein A affinity chromatography step to a flow-through purification step, the method comprising mixing the Protein A eluate with one or more virus inactivating agents using one or more in-line static mixers, wherein the flow of the Protein A eluate through the static mixer is in the laminar flow range comprising a Reynolds number below 100, wherein complete virus inactivation is achieved in less than 10 minutes or less than 5 minutes or less than 2 minutes or less than 1 minute.