US 9,809,798 B2
Pericyte progenitors from peripheral blood
Michael Raghunath, Singapore (SG); and Anna Blocki, Singapore (SG)
Assigned to National University of Singapore, Singapore (SG)
Appl. No. 14/4,349
Filed by Michael Raghunath, Singapore (SG); and Anna Blocki, Singapore (SG)
PCT Filed Mar. 12, 2012, PCT No. PCT/SG2012/000083
§ 371(c)(1), (2), (4) Date Sep. 10, 2013,
PCT Pub. No. WO2012/125123, PCT Pub. Date Sep. 20, 2012.
Claims priority of provisional application 61/451,637, filed on Mar. 11, 2011.
Prior Publication US 2014/0004046 A1, Jan. 2, 2014
Int. Cl. C12N 5/071 (2010.01); A61K 38/00 (2006.01)
CPC C12N 5/069 (2013.01) [C12N 5/0692 (2013.01); A61K 38/00 (2013.01); C12N 2500/38 (2013.01); C12N 2502/28 (2013.01); C12N 2533/52 (2013.01); C12N 2533/70 (2013.01); C12N 2533/90 (2013.01)] 21 Claims
1. A method of obtaining pericyte progenitor cells comprising:
a) culturing blood cells with a matrix to which cells can attach, and one or more carbohydrate-based hydrophilic macromolecules, each of the one or more of the macromolecules has a molecular weight from about 50 kDa to about 1000 kDa, and wherein the one or more macromolecules are present at a concentration from about 2.5 mg/ml to about 100 mg/ml, thereby producing a cell culture; and
b) maintaining the cell culture under conditions in which pericyte progenitor cells are generated in the cell culture, wherein the pericyte progenitor cells express platelet-derived growth factor receptor-β (PDGFR-β) and neuron-glial antigen 2 (NG2),
c) thereby obtaining pericyte progenitor cells.