US 9,809,796 B2
Animal protein-free media for cultivation of cells
Leopold Gillberger, Vienna (AT); Manfred Reiter, Vienna (AT); Wolfgang Mundt, Vienna (AT); and Friedrich Dorner, Vienna (AT)
Assigned to Baxalta GmbH, Glattpark (Opfikon) (CH); and Baxalta Incorporated, Bannockburn, IL (US)
Filed by Baxalta Incorporated, Bannockburn, IL (US); and Baxalta GmbH, Glattpark (Opfikon) (CH)
Filed on May 18, 2017, as Appl. No. 15/598,406.
Application 11/858,844 is a division of application No. 10/976,399, filed on Oct. 29, 2004, abandoned.
Application 15/598,406 is a continuation of application No. 14/954,705, filed on Nov. 30, 2015, granted, now 9,714,411.
Application 14/954,705 is a continuation of application No. 14/268,859, filed on May 2, 2014, granted, now 9,222,075, issued on Dec. 29, 2015.
Application 14/268,859 is a continuation of application No. 13/864,118, filed on Apr. 16, 2013, granted, now 8,748,156, issued on Jun. 10, 2014.
Application 13/864,118 is a continuation of application No. 12/965,111, filed on Dec. 10, 2010, granted, now 8,440,408, issued on May 14, 2013.
Application 12/965,111 is a continuation of application No. 11/858,844, filed on Sep. 20, 2007, abandoned.
Prior Publication US 2017/0267969 A1, Sep. 21, 2017
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/20 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC C12N 5/0043 (2013.01) [C12N 5/0682 (2013.01); C12N 2500/46 (2013.01); C12N 2500/74 (2013.01); C12N 2500/76 (2013.01); C12N 2500/92 (2013.01)] 19 Claims
 
1. A method for expressing a target protein, comprising the steps of:
a) providing a culture of cells into which a nucleic acid comprising a sequence encoding the target protein has been introduced;
b) culturing the cells in an animal protein-free cell culture medium comprising putrescine and at least one protein hydrolysate, wherein the hydrolysate is derived from a plant or yeast, and wherein the putrescine is present in the animal protein-free cell culture medium in a concentration ranging from about 0.5 to about 10 mg/L.