US 9,809,792 B2
One-way separator for retaining and recirculating cells
Joerg Kauling, Bergisch Gladbach (DE); Juri Seletzky, Berkeley, CA (US); Jorgen Magnus, Dusseldorf (DE); Andre Pastor, Solingen (DE); and Helmut Brod, Cologne (DE)
Assigned to BAYER AKTIENGESELLSCHAFT, Leverkusen (DE)
Appl. No. 14/379,577
Filed by BAYER AKTIENGESELLSCHAFT, Leverkusen (DE)
PCT Filed Feb. 20, 2013, PCT No. PCT/EP2013/053390
§ 371(c)(1), (2) Date Aug. 19, 2014,
PCT Pub. No. WO2013/124326, PCT Pub. Date Aug. 29, 2013.
Claims priority of application No. 12001121 (EP), filed on Feb. 20, 2012.
Prior Publication US 2015/0017716 A1, Jan. 15, 2015
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/00 (2006.01); C12M 3/06 (2006.01)
CPC C12M 33/22 (2013.01) [C12M 23/06 (2013.01); C12M 23/14 (2013.01); C12M 23/26 (2013.01); C12M 23/28 (2013.01); C12M 27/16 (2013.01); C12M 29/04 (2013.01); C12M 47/02 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A solids separator for retaining and recirculating solids from a reactor mixture, comprising a flow-bearing sterilizable plastic bag or plastic bottle and, within the plastic bag or plastic bottle:
in the upper region, one or more passages/internals for withdrawing from a harvest stream collection region a harvest stream that is separated from the solids,
in the upper segment of a central region, a separation region having a separation area, which, during operation, is inclined at an angle of 0° to 80° to the horizontal,
in the lower segment of the central region, one or more passages or internals for a uniform horizontal flow distribution of the reactor mixture over an introduction surface, wherein the separation area is situated above the introduction surface,
in the lower region a solids collection region that is tapered at the bottom for collecting the solids using gravity.