US 9,809,642 B2
Transgenic chicken comprising an inactivated immunoglobulin gene
Philip A. Leighton, San Francisco, CA (US); William Don Harriman, Alameda, CA (US); and Robert Etches, Oakland, CA (US)
Assigned to Crystal Bioscience Inc., Emeryville, CA (US)
Filed by Crystal Bioscience Inc., Emeryville, CA (US)
Filed on May 27, 2016, as Appl. No. 15/167,876.
Application 15/167,876 is a continuation of application No. 14/114,159, granted, now 9,380,769, previously published as PCT/US2012/039191, filed on May 23, 2012.
Claims priority of provisional application 61/489,638, filed on May 24, 2011.
Prior Publication US 2016/0297871 A1, Oct. 13, 2016
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/00 (2006.01); A01K 67/027 (2006.01); C12N 15/90 (2006.01)
CPC C07K 16/00 (2013.01) [A01K 67/0276 (2013.01); C12N 15/902 (2013.01); A01K 2217/075 (2013.01); A01K 2217/15 (2013.01); A01K 2227/30 (2013.01); C07K 2317/14 (2013.01); C07K 2317/24 (2013.01); C07K 2317/51 (2013.01)] 11 Claims
1. A chicken primordial germ cell having a genome in which the endogenous heavy chain immunoglobulin locus has been modified by homologous recombination to contain a selectable marker in place of the endogenous JH region of the locus,
wherein the sequences that flank the selectable marker in the modified heavy chain immunoglobulin locus are the same as or amplified from the chicken primordial germ cell prior to modification.