US 9,809,639 B2
Purification of biological products by constrained cohydration chromatography
Peter Stanley Gagnon, Singapore (SG)
Assigned to AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH, Singapore (SG)
Appl. No. 14/124,706
Filed by Peter Stanley Gagnon, Singapore (SG)
PCT Filed Jun. 1, 2012, PCT No. PCT/SG2012/000199
§ 371(c)(1), (2), (4) Date Apr. 28, 2014,
PCT Pub. No. WO2012/169970, PCT Pub. Date Dec. 13, 2012.
Claims priority of provisional application 61/494,669, filed on Jun. 8, 2011.
Claims priority of provisional application 61/494,687, filed on Jun. 8, 2011.
Claims priority of provisional application 61/653,950, filed on May 31, 2012.
Claims priority of application No. 201108397 (SG), filed on Nov. 14, 2011.
Prior Publication US 2014/0227766 A1, Aug. 14, 2014
Int. Cl. G01N 33/53 (2006.01); C12Q 1/70 (2006.01); C12Q 1/02 (2006.01); C12M 1/00 (2006.01); C07K 14/75 (2006.01); B01D 15/16 (2006.01); C07K 1/16 (2006.01); C07K 16/06 (2006.01); B01D 15/08 (2006.01); C07K 14/755 (2006.01); C12N 7/00 (2006.01); B01D 15/18 (2006.01); B01D 15/38 (2006.01)
CPC C07K 14/75 (2013.01) [B01D 15/08 (2013.01); B01D 15/166 (2013.01); C07K 1/16 (2013.01); C07K 14/755 (2013.01); C07K 16/065 (2013.01); C12N 7/00 (2013.01); B01D 15/1807 (2013.01); B01D 15/3885 (2013.01); C12N 2795/00 (2013.01)] 36 Claims
OG exemplary drawing
 
1. A method for purification of a hydrated target species of biological origin in a sample comprising the steps of contacting the sample with a hydrated surface of an undissolved material and contacting the sample with a constraining agent in an amount sufficient to cause more than 50% to substantially all of the target species to be retained at the hydrated surface of the undissolved material, wherein
(i) the undissolved material comprises particles,
(ii) a surface of the particles includes charged moieties,
(iii) the target species is retained exclusively by constrained cohydration under conditions providing a substantial absence of a direct chemical interaction between the target species and the hydrated surface, and
(iv) the conditions that provide the substantial absence of a direct chemical interaction comprise pH, conductivity and salt concentration of buffers delivered to a column of the particles, wherein said conditions discourage direct interaction between the hydrated target species and the charged moieties, and wherein the particles are optionally a convective chromatography material.