US 9,809,632 B2
Universal protein tag for double stranded nucleic acid delivery
Xiaohu Gao, Seattle, WA (US); and Hong Yan Liu, Seattle, WA (US)
Assigned to University of Washington Through its Center for Commercialization, Seattle, WA (US)
Appl. No. 14/914,922
Filed by UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION, Seattle, WA (US)
PCT Filed Oct. 22, 2014, PCT No. PCT/US2014/061714
§ 371(c)(1), (2) Date Feb. 26, 2016,
PCT Pub. No. WO2015/061409, PCT Pub. Date Apr. 30, 2015.
Claims priority of provisional application 61/894,806, filed on Oct. 23, 2013.
Prior Publication US 2016/0222072 A1, Aug. 4, 2016
Int. Cl. C07K 14/47 (2006.01); A61K 38/17 (2006.01); A61K 31/713 (2006.01); C12N 15/11 (2006.01); C12N 15/87 (2006.01)
CPC C07K 14/47 (2013.01) [A61K 31/713 (2013.01); A61K 38/1709 (2013.01); C07K 14/4703 (2013.01); C12N 15/111 (2013.01); C12N 15/87 (2013.01); C07K 2319/21 (2013.01); C07K 2319/80 (2013.01); C07K 2319/85 (2013.01); C12N 2310/14 (2013.01); C12N 2310/16 (2013.01); C12N 2310/3519 (2013.01); C12N 2320/32 (2013.01)] 12 Claims
 
1. A chimeric protein, comprising:
(a) one or more double stranded ribonucleic acid binding domains (dsRBDs), wherein the one or more dsRBDs comprise the amino acid sequence selected from the group consisting of SEQ ID NOs: 2-36 and 38-80; and
(b) one or more polyHis domains, wherein each polyHis domain comprises at least 6 consecutive histidine residues, and wherein the one or more polyHis domains in total consist of 18 histidine residues.