US 9,809,628 B2
Modular DNA-binding domains and methods of use
Ulla Bonas, Halle (DE); Jens Boch, Wunstorf (DE); Sebastian Schornack, Cambridge (GB); and Thomas Lahaye, Mössingen (DE)
Filed by Ulla Bonas, Halle (DE); Jens Boch, Wunstorf (DE); Sebastian Schornack, Cambridge (GB); and Thomas Lahaye, Mössingen (DE)
Filed on Jul. 28, 2016, as Appl. No. 15/222,498.
Application 15/222,498 is a division of application No. 14/625,698, filed on Feb. 19, 2015, granted, now 9,453,054.
Application 14/625,698 is a continuation of application No. 14/153,241, filed on Jan. 13, 2014, granted, now 9,017,967, issued on Apr. 28, 2015.
Application 14/153,241 is a continuation of application No. 13/755,826, filed on Jan. 31, 2013, abandoned.
Application 13/755,826 is a continuation of application No. 13/019,526, filed on Feb. 2, 2011, abandoned.
Application 13/019,526 is a continuation in part of application No. 13/016,297, filed on Jan. 28, 2011, granted, now 9,353,378, issued on May 31, 2016.
Application 13/016,297 is a continuation of application No. PCT/IB2010/000154, filed on Jan. 12, 2010.
Claims priority of provisional application 61/225,043, filed on Jul. 13, 2009.
Claims priority of application No. 10 2009 004 659 (DE), filed on Jan. 12, 2009; and application No. 09165328 (EP), filed on Jul. 13, 2009.
Prior Publication US 2017/0051020 A1, Feb. 23, 2017
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/195 (2006.01); C12N 15/10 (2006.01); C12N 15/82 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01); C12N 1/20 (2006.01); C12N 5/071 (2010.01); C07H 21/04 (2006.01); C07K 14/00 (2006.01); C12N 5/04 (2006.01)
CPC C07K 14/195 (2013.01) [C07H 21/04 (2013.01); C07K 14/001 (2013.01); C12N 1/20 (2013.01); C12N 5/04 (2013.01); C12N 5/0602 (2013.01); C12N 15/1034 (2013.01); C12N 15/825 (2013.01); C12N 15/8216 (2013.01); C12N 15/8217 (2013.01); C12N 15/8238 (2013.01); C12N 15/8239 (2013.01); C12N 15/8279 (2013.01); C12Q 1/6816 (2013.01); G01N 33/5308 (2013.01); C07K 2319/09 (2013.01); C07K 2319/60 (2013.01); C07K 2319/80 (2013.01); C12N 2501/998 (2013.01)] 15 Claims
 
1. A protein comprising an artificial transcription activator-like (TAL) effector repeat region,
wherein the artificial TAL effector repeat region comprises a modified TAL effector repeat domain comprising a plurality of contiguous repeat units, and
wherein the protein is capable of recognizing a predetermined nucleotide sequence in an animal cell.