US 9,809,614 B2
Synthetic membrane anchors
Nicolai Bovin, Moscow (RU); Lissa Gilliver, Auckland (NZ); Stephen Henry, Auckland (NZ); and Elena Korchagina, Moscow (RU)
Assigned to KODE BIOTECH LIMITED, Auckland (NZ)
Filed by KODE BIOTECH LIMITED, Auckland (NZ)
Filed on May 30, 2016, as Appl. No. 15/168,144.
Application 13/067,021 is a division of application No. 10/593,829, granted, now 8,013,131, issued on Sep. 6, 2011, previously published as PCT/NZ2005/000052, filed on Mar. 22, 2005.
Application 15/168,144 is a continuation of application No. 14/108,749, filed on Dec. 17, 2013, granted, now 9,353,349, issued on May 31, 2016.
Application 14/108,749 is a continuation of application No. 13/067,021, filed on May 3, 2011, granted, now 8,637,473, issued on Jan. 28, 2014.
Claims priority of application No. 531866 (NZ), filed on Mar. 22, 2004; and application No. 537941 (NZ), filed on Jan. 28, 2005.
Prior Publication US 2016/0333044 A1, Nov. 17, 2016
Int. Cl. C07H 15/10 (2006.01); C07H 1/00 (2006.01); C07F 9/10 (2006.01); C07H 15/04 (2006.01); C12N 5/00 (2006.01); C12N 5/073 (2010.01); C12N 5/078 (2010.01); A61K 35/12 (2015.01)
CPC C07H 15/10 (2013.01) [C07F 9/10 (2013.01); C07H 1/00 (2013.01); C07H 15/04 (2013.01); C12N 5/0006 (2013.01); C12N 5/0604 (2013.01); C12N 5/0641 (2013.01); A61K 2035/124 (2013.01)] 4 Claims
 
1. A method of preparing a synthetic molecule construct of the structure F—S1—S2-L, comprising the steps of:
reacting a bis(succinimidyl)dicarboxylate with a phosphatidylethanolamine to provide a phosphatidylethanolamide derivative of the structure S2-L; and then
reacting a primary aminoalkyl glycoside of the structure F—S1 with the phosphatidylethanolamide derivative to provide the construct,
where F is a mono-, di-, tri- or oligo-saccharide; S1 is 2-aminoethyl, 3-aminopropyl, 4-aminobutyl, or 5-aminopentyl; S2 is —CO(CH2)2CO—, —CO(CH2)3CO—, —CO(CH2)4CO— or —CO(CH2)5CO—; and L is the phosphatidylethanolamide.