US 9,809,612 B2
Method for isolating nucleic acids
Christoph Ritt, Langenfeld (DE); Martin Horlitz, Düsseldorf (DE); and Markus Sprenger-Haussels, Mettmann (DE)
Assigned to QIAGEN GmbH, Hilden (DE)
Appl. No. 12/995,348
Filed by Christoph Ritt, Langenfeld (DE); Martin Horlitz, Düsseldorf (DE); and Markus Sprenger-Haussels, Mettmann (DE)
PCT Filed May 12, 2009, PCT No. PCT/EP2009/003364
§ 371(c)(1), (2), (4) Date Feb. 10, 2011,
PCT Pub. No. WO2009/146776, PCT Pub. Date Dec. 10, 2009.
Claims priority of application No. 08009941 (EP), filed on May 30, 2008.
Prior Publication US 2011/0130558 A1, Jun. 2, 2011
Int. Cl. C07H 1/08 (2006.01); C12N 15/10 (2006.01)
CPC C07H 1/08 (2013.01) [C12N 15/1006 (2013.01)] 39 Claims
 
1. A method for isolating or purifying short-chain nucleic acids that is at most 500 bp from a short-chain nucleic acid-containing starting material, comprising:
(a) binding the short-chain nucleic acids to a nucleic acid-binding support material by contacting the starting material with said nucleic acid-binding support material in the presence of at least one chaotropic compound and at least one branched or unbranched alcohol, said alcohol being present at a concentration of at least 15% (v/v) and at most 25% (v/v), wherein a non-ionic detergent is present during binding,
wherein the short-chain nucleic acids are extracellular DNA, and
wherein the short-chain nucleic acid-containing starting material is selected from plasma, serum, a bodily fluid that does not contain cells, and a bodily fluid that is prepared not to contain cells.