US 9,808,802 B2
Microfluidic device for serial fluidic operations
Pamela Ann Dothie, Chilton (GB); and Daniel Christopher Spencer, Hampshire (GB)
Assigned to SHARP LIFE SCIENCE (EU) LIMITED, Oxford (GB)
Filed by Sharp Life Science (EU) Limited, Oxford (GB)
Filed on Aug. 12, 2016, as Appl. No. 15/235,677.
Application 15/235,677 is a division of application No. 13/963,215, filed on Aug. 9, 2013, granted, now 9,440,233.
Prior Publication US 2016/0346782 A1, Dec. 1, 2016
Int. Cl. B01L 3/00 (2006.01); F16K 99/00 (2006.01); G01N 33/49 (2006.01); G01N 33/569 (2006.01); G01N 15/14 (2006.01); C07C 231/02 (2006.01); G01N 33/80 (2006.01)
CPC B01L 3/502738 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); C07C 231/02 (2013.01); F16K 99/0013 (2013.01); F16K 99/0028 (2013.01); G01N 15/1404 (2013.01); G01N 33/4915 (2013.01); G01N 33/56972 (2013.01); G01N 33/80 (2013.01); B01L 2200/0605 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0644 (2013.01); F16K 2099/0084 (2013.01); F16K 2099/0086 (2013.01); Y10T 137/0318 (2015.04); Y10T 137/85986 (2015.04); Y10T 137/86863 (2015.04); Y10T 137/86919 (2015.04)] 15 Claims
OG exemplary drawing
 
1. An integrated microfluidic device for carrying out a series of fluidic operations comprising:
a housing including a plurality of n microfluidic conduits, wherein n is at least three; and
a rotating valve having an internal channel with an entrance port and an exit port that are angularly separated;
wherein the rotating valve is positionable in a first position to connect two of the n fluidic conduits via the internal channel, and upon rotating the valve to a second position, two of the n fluidic conduits, including at least one fluidic conduit different from the fluidic conduits connected in the first position, are connected by the internal channel; and
wherein in either the first position or the second position, two sequentially adjacent fluidic conduits are connected via the internal channel;
further comprising a first fluidic chamber in fluid communication with one of the fluidic conduits, wherein fluid contained in the first fluidic chamber is transferrable by application of positive or negative displacement pressure from the first fluidic chamber through the one of the fluidic conduits, and via the entrance port into the internal channel;
wherein the fluid is transferrable by the application of the positive or negative displacement pressure from the internal channel via the exit port into a second one of the fluidic conduits;
further comprising a second fluidic chamber in fluid communication with the second one of the fluidic conduits, wherein the fluid is transferrable from the second one of the fluidic conduits into the second fluidic chamber; and
wherein the first fluidic chamber includes a first moveable piston that applies the positive or negative displacement pressure to transfer the fluid from the first fluidic chamber.