US 9,808,799 B2
Apparatus for and method of processing biological samples
Joseph Amshey, Encinitas, CA (US); Daniel Bezdek, Oceanside, CA (US); Espir Kahatt, Carlsbad, CA (US); Alexander Khorlin, Mountain View, CA (US); Lance Parsons, San Marcos, CA (US); Todd Peterson, Coronado, CA (US); Timothy Powers, Carlsbad, CA (US); Michael Thacker, San Diego, CA (US); Timothy Updyke, Temecula, CA (US); and Kornelija Zgonc, Carlsbad, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Aug. 19, 2014, as Appl. No. 14/462,640.
Application 14/462,640 is a continuation of application No. 13/615,500, filed on Sep. 13, 2012, granted, now 8,845,984.
Application 13/615,500 is a continuation of application No. 12/549,311, filed on Aug. 27, 2009, granted, now 8,404,198, issued on Mar. 26, 2013.
Claims priority of provisional application 61/139,539, filed on Dec. 19, 2008.
Claims priority of provisional application 61/108,019, filed on Oct. 23, 2008.
Claims priority of provisional application 61/098,586, filed on Sep. 19, 2008.
Claims priority of provisional application 61/092,338, filed on Aug. 27, 2008.
Prior Publication US 2015/0044758 A1, Feb. 12, 2015
Int. Cl. B01L 9/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/50273 (2013.01); B01L 7/00 (2013.01); B01L 9/527 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/088 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0605 (2013.01); B01L 2400/0655 (2013.01); G01N 2035/00306 (2013.01); G01N 2035/1037 (2013.01); Y10T 436/2575 (2015.01)] 12 Claims
OG exemplary drawing
 
1. A bioprocessing cartridge comprising:
a first bioprocessing chamber, wherein the first bioprocessing chamber includes first solid support, and wherein the first solid support comprises a cell lysate clarification filter;
a second bioprocessing chamber, wherein the second bioprocessing chamber has a fluid volume between about 250 μl and about 100 ml and includes a second solid support, wherein the second solid support comprises a plurality of magnetic beads;
a plurality of fluid channels in fluid communication with the at least one bioprocessing chamber, wherein at least two of the plurality of fluid channels have differentially sized diameters;
at least one access valve, wherein the access valve is placed within a flow path between a fluid connector and one of the plurality of fluid channels, each fluid connector configured to fluidly connect the cartridge to at least one fluid container; and
at least one control fluid connector, wherein the at least one control fluid connector is configured to form a sealed connection with a control fluid manifold and to provide a control fluid to actuate the at least one access valve.