US 9,808,799 B2
Apparatus for and method of processing biological samples
Joseph Amshey, Encinitas, CA (US); Daniel Bezdek, Oceanside, CA (US); Espir Kahatt, Carlsbad, CA (US); Alexander Khorlin, Mountain View, CA (US); Lance Parsons, San Marcos, CA (US); Todd Peterson, Coronado, CA (US); Timothy Powers, Carlsbad, CA (US); Michael Thacker, San Diego, CA (US); Timothy Updyke, Temecula, CA (US); and Kornelija Zgonc, Carlsbad, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed on Aug. 19, 2014, as Appl. No. 14/462,640.
Application 14/462,640 is a continuation of application No. 13/615,500, filed on Sep. 13, 2012, granted, now 8,845,984.
Application 13/615,500 is a continuation of application No. 12/549,311, filed on Aug. 27, 2009, granted, now 8,404,198, issued on Mar. 26, 2013.
Claims priority of provisional application 61/139,539, filed on Dec. 19, 2008.
Claims priority of provisional application 61/108,019, filed on Oct. 23, 2008.
Claims priority of provisional application 61/098,586, filed on Sep. 19, 2008.
Claims priority of provisional application 61/092,338, filed on Aug. 27, 2008.
Prior Publication US 2015/0044758 A1, Feb. 12, 2015
Int. Cl. B01L 9/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/50273 (2013.01); B01L 7/00 (2013.01); B01L 9/527 (2013.01); B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/088 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0605 (2013.01); B01L 2400/0655 (2013.01); G01N 2035/00306 (2013.01); G01N 2035/1037 (2013.01); Y10T 436/2575 (2015.01)] 12 Claims
OG exemplary drawing
1. A bioprocessing cartridge comprising:
a first bioprocessing chamber, wherein the first bioprocessing chamber includes first solid support, and wherein the first solid support comprises a cell lysate clarification filter;
a second bioprocessing chamber, wherein the second bioprocessing chamber has a fluid volume between about 250 μl and about 100 ml and includes a second solid support, wherein the second solid support comprises a plurality of magnetic beads;
a plurality of fluid channels in fluid communication with the at least one bioprocessing chamber, wherein at least two of the plurality of fluid channels have differentially sized diameters;
at least one access valve, wherein the access valve is placed within a flow path between a fluid connector and one of the plurality of fluid channels, each fluid connector configured to fluidly connect the cartridge to at least one fluid container; and
at least one control fluid connector, wherein the at least one control fluid connector is configured to form a sealed connection with a control fluid manifold and to provide a control fluid to actuate the at least one access valve.