US 9,808,558 B2
Allografts combined with tissue derived stem cells for bone healing
Yaling Shi, Larkspur, CO (US)
Assigned to AlloSource, Centennial, CO (US)
Filed by AlloSource, Centennial, CO (US)
Filed on Oct. 12, 2015, as Appl. No. 14/880,675.
Application 14/880,675 is a continuation of application No. 12/612,583, filed on Nov. 4, 2009, granted, now 9,192,695.
Claims priority of provisional application 61/116,484, filed on Nov. 20, 2008.
Prior Publication US 2016/0045640 A1, Feb. 18, 2016
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/38 (2015.01); A61L 27/38 (2006.01); A61L 27/36 (2006.01); A61L 27/54 (2006.01); C12N 5/0775 (2010.01); A01N 1/02 (2006.01); A61K 35/12 (2015.01)
CPC A61L 27/3834 (2013.01) [A01N 1/021 (2013.01); A61K 35/38 (2013.01); A61L 27/3608 (2013.01); A61L 27/3847 (2013.01); A61L 27/3895 (2013.01); A61L 27/54 (2013.01); C12N 5/0662 (2013.01); C12N 5/0667 (2013.01); A61K 35/12 (2013.01); A61L 2300/406 (2013.01); A61L 2430/02 (2013.01); C12N 2509/00 (2013.01); C12N 2533/18 (2013.01); C12N 2533/90 (2013.01)] 24 Claims
 
1. A method of making an allograft product for enhancing bone formation, the method consisting of:
providing a bone substrate obtained from a human, cadaveric donor;
providing a tissue comprising mesenchymal stem cells and unwanted cells from the human, cadaveric donor;
processing the tissue to form a cell suspension comprising the mesenchymal stem cells and the unwanted cells;
adding the cell suspension to the bone substrate to form a seeded bone substrate;
incubating the seeded bone substrate for a period of time to allow the mesenchymal stem cells to adhere to the bone substrate; and
rinsing the seeded bone substrate to remove the unwanted cells from the bone substrate;
thereby making the allograft product for enhancing bone formation, wherein the allograft product comprises bone substrate with mesenchymal stem cells adhered thereto.