US 9,808,536 B2
Hypericin albumin nanoparticle-Escherichia coli serum antibody complex and preperation method and application thereof
Jianping Liang, Lanzhou (CN); Wenzhu Guo, Lanzhou (CN); Baocheng Hao, Lanzhou (CN); Lei Tao, Lanzhou (CN); Yu Liu, Lanzhou (CN); Xuehong Wang, Lanzhou (CN); Ruofeng Shang, Lanzhou (CN); Zhiting Guo, Lanzhou (CN); Fengwu Zhao, Lanzhou (CN); Zhen Yang, Lanzhou (CN); Hong Chen, Lanzhou (CN); and Zhong Jia, Lanzhou (CN)
Assigned to LANZHOU INSTITUTE OF HUSBANDRY AND PHARMACEUTICAL SCIENCES OF CAAS, Lanzhou (CN)
Filed by LANZHOU INSTITUTE OF HUSBANDRY AND PHARMACEUTICAL SCIENCES OF CAAS, Lanzhou, Gansu Province (CN)
Filed on Mar. 25, 2016, as Appl. No. 15/81,657.
Claims priority of application No. 2015 1 0223043 (CN), filed on May 5, 2015.
Prior Publication US 2016/0324978 A1, Nov. 10, 2016
Int. Cl. A61K 39/00 (2006.01); A61K 39/38 (2006.01); A61K 39/108 (2006.01); A61K 47/48 (2006.01); C07K 16/12 (2006.01); C07K 14/76 (2006.01)
CPC A61K 47/48507 (2013.01) [A61K 47/48884 (2013.01); C07K 14/76 (2013.01); C07K 16/1232 (2013.01); A61K 2039/505 (2013.01)] 4 Claims
 
1. A hypericin albumin nanoparticle-Escherichia coli serum antibody complex is characterized by being prepared through the following steps:
1) mixing hypericin albumin nanoparticles and rabbit anti-Escherichia coli to obtain a mixed liquor;
2) adding the mixed liquor from Step 1) into ethanediol to obtain a ethanediol mixed liquor;
3) irradiating the ethanediol mixed liquor from Step 2) with carbon ion, placing the mixed liquor at −2° C.-5° C. for 2 hours after irradiation to obtain an irradiated ethanediol mixed liquor;
4) centrifuging the irradiated ethanediol mixed liquor from Step 3) at a low temperature, discarding a supernatant, and freezing and drying precipitates to obtain the hypericin albumin nanoparticle-Escherichia coli serum antibody complex,
wherein the hypericin albumin nanoparticles and the rabbit anti-Escherichia coli are mixed according to the ratio of (800-1600) mg:(4-10) μg in Step 1);
wherein the ratio of hypericin albumin nanoparticles:ethanediol is (800-1600) mg (12-30) g in Step 2);
wherein the irradiation dose of carbon ion is 3-10 Gy in Step 3); and
wherein the temperature of the low temperature centrifugation is −2° C.-5° C., the centrifugal rotational speed is 16500 r/min and the cryogenic temperature of precipitates is −2-0° C. in Step 4).