US 9,808,532 B2
Peptides whose uptake in cells is controllable
Roger Tsien, La Jolla, CA (US); Emilia Olson, Seattle, WA (US); Tao Jiang, San Diego, CA (US); Quyen Nguyen, Del Mar, CA (US); and Michael Whitney, San Diego, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 13/384,581
Filed by Roger Tsien, La Jolla, CA (US); Emilia Olson, Seattle, WA (US); Tao Jiang, San Diego, CA (US); Quyen Nguyen, Del Mar, CA (US); and Michael Whitney, San Diego, CA (US)
PCT Filed Jul. 15, 2010, PCT No. PCT/US2010/042188
§ 371(c)(1), (2), (4) Date Feb. 16, 2012,
PCT Pub. No. WO2011/008996, PCT Pub. Date Jan. 20, 2011.
Claims priority of provisional application 61/225,872, filed on Jul. 15, 2009.
Prior Publication US 2012/0134922 A1, May 31, 2012
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 10/00 (2006.01); A61B 5/00 (2006.01); A61B 8/00 (2006.01); A61K 47/48 (2006.01); A61K 49/00 (2006.01); C07K 7/06 (2006.01)
CPC A61K 47/48215 (2013.01) [A61K 47/48207 (2013.01); A61K 47/48238 (2013.01); A61K 47/48815 (2013.01); A61K 49/0032 (2013.01); A61K 49/0056 (2013.01); C07K 7/06 (2013.01)] 11 Claims
 
1. A molecule having the formula: (A-X-B-C)n-M wherein:
C is a fluorescent moiety;
A is a peptide with a sequence comprising a series of 5 glutamate residues;
B is a peptide with a sequence comprising a series of 8 arginine residues;
X is a cleavable linker comprising PRSFL (SEQ ID NO: 22);
M is a macromolecular carrier; and
n is an integer between 1 and 20;
wherein M is bound to A or B.