US 9,808,492 B2
Methods of preparing lyophilized human tissues
Terry W. Broussard, The Woodlands, TX (US)
Filed by Terry W. Broussard, The Woodlands, TX (US)
Filed on Jul. 16, 2015, as Appl. No. 14/801,747.
Application 14/801,747 is a continuation of application No. 13/608,475, filed on Sep. 10, 2012, abandoned.
Prior Publication US 2015/0320906 A1, Nov. 12, 2015
Int. Cl. A61L 27/36 (2006.01); A61K 35/50 (2015.01); A61L 27/50 (2006.01); A61L 27/60 (2006.01); C12N 5/073 (2010.01)
CPC A61K 35/50 (2013.01) [A61L 27/3604 (2013.01); A61L 27/3641 (2013.01); A61L 27/3662 (2013.01); A61L 27/3679 (2013.01); A61L 27/3691 (2013.01); A61L 27/507 (2013.01); A61L 27/60 (2013.01); C12N 5/0605 (2013.01); A61L 2430/10 (2013.01); A61L 2430/34 (2013.01)] 15 Claims
 
1. A method of preparing a tissue to be a biological dressing or a tissue graft, said method comprising, in listed order:
processing and cleaning, consisting of using only a physiologic saline solution and physical manipulation, an amniotic membrane maintaining the following layers in the following order:
a) an epithelium layer;
b) a basement membrane layer; and
c) a stroma layer comprising an upper compact layer, a middle fibroblast layer, and a lower spongy layer;
freezing and dehydrating while lyophilizing, without heat or chemicals, the amniotic membrane after said cleaning to create a frozen, dehydrated amniotic membrane; and
in a frozen state, sterilizing the frozen, dehydrated amniotic membrane using gamma irradiation.